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Analysis of FAM65A interactions

ABSTRACT: We identified FAM65A as a novel interactor of RHOA. To reveal other binding partners of FAM65A, we carried out a quantitative IP-MS, using SILAC labelled HeLa cells ectopically expressing GFP-FAM65A or GFP alone as control. Experiments were performed in duplicate with switched labelling and specific interactors of FAM65A were revealed from background binders using the heavy to light SILAC enrichments.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Cell Culture

DISEASE(S): Cervix Carcinoma

SUBMITTER: Faraz Mardakheh

LAB HEAD: Christopher J Marshall

PROVIDER: PXD004933 | Pride | 2016-11-01

REPOSITORIES: Pride

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