Ontology highlight
ABSTRACT:
ORGANISM(S): Mus musculus
SUBMITTER: Johannes Beckers
PROVIDER: E-GEOD-51605 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Pertek Anna A Meier Florian F Irmler Martin M Beckers Johannes J Skylaki Stavroula S Endele Max M Wurst Wolfgang W Prakash Nilima N Kühn Ralf R
Molecular biotechnology 20140801 8
Mammalian cells can be reprogrammed into induced pluripotent stem cells (iPSCs), a valuable tool for in vitro disease modeling and regenerative medicine. These applications demand for iPSCs devoid of reprogramming factor transgenes, but current procedures for the derivation of transgene-free iPSCs are inefficient and cumbersome. Here, we describe a new approach for the simple derivation of transgene-free iPSCs by the sequential use of two DNA recombinases, C31 Integrase and Cre, to control the g ...[more]