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Gene expression in third instar larval eye discs

ABSTRACT: We used microarrays to identify genes that are differentially expressed in the absence of miR-998 expression. Drosophila third instar larvae were selected for RNA extraction and hybridization on Affymetrix microarrays. Three biological replicates for each genotype were prepared. The data was normalized using the RMA algorithm.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Mary Truscott

PROVIDER: E-GEOD-51724 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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An intronic microRNA links Rb/E2F and EGFR signaling.

Truscott Mary M Islam Abul B M M K AB Lightfoot James J López-Bigas Núria N Frolov Maxim V MV

PLoS genetics 20140724 7

The importance of microRNAs in the regulation of various aspects of biology and disease is well recognized. However, what remains largely unappreciated is that a significant number of miRNAs are embedded within and are often co-expressed with protein-coding host genes. Such a configuration raises the possibility of a functional interaction between a miRNA and the gene it resides in. This is exemplified by the Drosophila melanogaster dE2f1 gene that harbors two miRNAs, mir-11 and mir-998, within ...[more]

PMID: 25058496

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