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Global Expression profiling of mutant mouse multipotent progenitors.

ABSTRACT: Global Expression profiling of mutant mouse multipotent progenitors was performed. In order to mitigate the impact of the studied driver mutations on cell surface phenotypes, we performed transcriptome analysis on a homogeneous population of purified lineage negative, Sca-1/Kit positive multipotent progenitor cells (MPPs, cell surface profile: Lin-/CD34+/Flt3+/CD48+/CD150-). Aprroximately 1000 multipotent progenitors were isolated from wild type or mutant Npm1cA/+, NrasG12D, Npm1cA/+;NrasG12D, Flt3ITD/+ and Npm1cA/+;Flt3ITD/+ murine bone marrow cells. Total RNA extracted and mRNA amplified using

ORGANISM(S): Mus musculus

SUBMITTER: Oliver Dovey

PROVIDER: E-MTAB-5358 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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