Unknown,Transcriptomics,Genomics,Proteomics

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Identification of renal distal convoluted tubules enriched genes


ABSTRACT: The renal distal convoluted tubule DCT is a short part of the distal nephron with specific functions transporting ions and thereby modifying Na, Ca, Mg, K balance A transcriptomic analysis of the DCT was performed to identify specific gene expression which would implicate those genes in specific DCT function Fluorescent DCT (EGFP+) were sorted out from a renal tubule suspension by a COPAS (Complex Object Parametric analyser and sorter). EGFP- and the total (ALL) fractions were also sorted by COPAS. Gene expression for each fractions (EGFP+, EGFP-, ALL) was performed in 4 mice

ORGANISM(S): Mus musculus

SUBMITTER: Nicolas Picard 

PROVIDER: E-GEOD-51935 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Protein phosphatase 1 inhibitor-1 deficiency reduces phosphorylation of renal NaCl cotransporter and causes arterial hypotension.

Picard Nicolas N   Trompf Katja K   Yang Chao-Ling CL   Miller R Lance RL   Carrel Monique M   Loffing-Cueni Dominique D   Fenton Robert A RA   Ellison David H DH   Loffing Johannes J  

Journal of the American Society of Nephrology : JASN 20131114 3


The thiazide-sensitive NaCl cotransporter (NCC) of the renal distal convoluted tubule (DCT) controls ion homeostasis and arterial BP. Loss-of-function mutations of NCC cause renal salt wasting with arterial hypotension (Gitelman syndrome). Conversely, mutations in the NCC-regulating WNK kinases or kelch-like 3 protein cause familial hyperkalemic hypertension. Here, we performed automated sorting of mouse DCTs and microarray analysis for comprehensive identification of novel DCT-enriched gene pro  ...[more]

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