The budding yeast Centromere DNA Element II wraps a stable Cse4 hemisome in either orientation in vivo
Ontology highlight
ABSTRACT:
ORGANISM(S): Saccharomyces cerevisiae
SUBMITTER: Jorja Henikoff
PROVIDER: E-GEOD-51949 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Publications
The budding yeast Centromere DNA Element II wraps a stable Cse4 hemisome in either orientation in vivo.
Henikoff Steven S Ramachandran Srinivas S Krassovsky Kristina K Bryson Terri D TD Codomo Christine A CA Brogaard Kristin K Widom Jonathan J Wang Ji-Ping JP Henikoff Jorja G JG
eLife 20140415
In budding yeast, a single cenH3 (Cse4) nucleosome occupies the ∼120-bp functional centromere, however conflicting structural models for the particle have been proposed. To resolve this controversy, we have applied H4S47C-anchored cleavage mapping, which reveals the precise position of histone H4 in every nucleosome in the genome. We find that cleavage patterns at centromeres are unique within the genome and are incompatible with symmetrical structures, including octameric nucleosomes and (Cse4/ ...[more]
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