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Long noncoding RNAs are spatially correlated with transcription factors and regulate lung development

ABSTRACT: To identify potential biological functions for three lncRNAs (NANCI, LL12, and LL34) we used shRNAs to knockdown expression of lncRNAs in MLE12 cells, a cell resembling type two lung epithelial cells. This data set contains the microarrays looking at gene expression. We used biological triplicate for each shRNA treatment and each treatment group was compared to a biological triplicate of MLE12 cells treated with a scrambled shRNA.

ORGANISM(S): Mus musculus

SUBMITTER: Michael Morley

PROVIDER: E-GEOD-52389 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Long noncoding RNAs are spatially correlated with transcription factors and regulate lung development.

Herriges Michael J MJ Swarr Daniel T DT Morley Michael P MP Rathi Komal S KS Peng Tien T Stewart Kathleen M KM Morrisey Edward E EE

Genes & development 20140601 12

Long noncoding RNAs (lncRNAs) are thought to play important roles in regulating gene transcription, but few have well-defined expression patterns or known biological functions during mammalian development. Using a conservative pipeline to identify lncRNAs that have important biological functions, we identified 363 lncRNAs in the lung and foregut endoderm. Importantly, we show that these lncRNAs are spatially correlated with transcription factors across the genome. In-depth expression analyses of ...[more]

PMID: 24939938

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