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M. tuberculosis Rv3263 Knockout

ABSTRACT: We determine gene expression profile for the knockout of Rv3263 (DNA Methyltransferase) and compared to wildtype M. tuberculosis. Two RNA-Seq datasets are included: wildtype (H37Rv_SS1) and knockout (delta_Rv3263)

ORGANISM(S): Mycobacterium tuberculosis H37Rv

SUBMITTER: Thomas Ioerger

PROVIDER: E-GEOD-52551 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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DNA methylation impacts gene expression and ensures hypoxic survival of Mycobacterium tuberculosis.

Shell Scarlet S SS Prestwich Erin G EG Baek Seung-Hun SH Shah Rupal R RR Sassetti Christopher M CM Dedon Peter C PC Fortune Sarah M SM

PLoS pathogens 20130704 7

DNA methylation regulates gene expression in many organisms. In eukaryotes, DNA methylation is associated with gene repression, while it exerts both activating and repressive effects in the Proteobacteria through largely locus-specific mechanisms. Here, we identify a critical DNA methyltransferase in M. tuberculosis, which we term MamA. MamA creates N⁶-methyladenine in a six base pair recognition sequence present in approximately 2,000 copies on each strand of the genome. Loss of MamA reduces th ...[more]

PMID: 23853579

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