Ontology highlight
ABSTRACT:
ORGANISM(S): Mus musculus
SUBMITTER: Kenneth Paigen
PROVIDER: E-GEOD-52628 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Baker Christopher L CL Walker Michael M Kajita Shimpei S Petkov Petko M PM Paigen Kenneth K
Genome research 20140306 5
In mammals, genetic recombination during meiosis is limited to a set of 1- to 2-kb regions termed hotspots. Their locations are predominantly determined by the zinc finger protein PRDM9, which binds to DNA in hotspots and subsequently uses its SET domain to locally trimethylate histone H3 at lysine 4 (H3K4me3). This sets the stage for double-strand break (DSB) formation and reciprocal exchange of DNA between chromatids, forming Holliday junctions. Here we report genome-wide analyses of PRDM9-dep ...[more]