Unknown,Transcriptomics,Genomics,Proteomics

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ChIP-seq analyses of 10 quorum sensing regulators in Pseudomonas aeruginosa


ABSTRACT: The Pseudomonas aeruginosa quorum-sensing (QS) systems contribute to bacterial homeostasis and pathogenicity. Although many regulators have been characterized to control the production of virulence factors and QS signaling molecules, its detailed regulatory mechanisms still remain elusive. Here, we performed chromatin immunoprecipitation followed by high-throughput DNA sequencing (ChIP-seq) on 10 key QS regulators. The direct regulation of these genes by corresponding regulator has been confirmed by Electrophoretic mobility shift assays (EMSAs) and quantitative real-time polymerase chain reactions (qRT-PCR). Binding motifs are found by using MEME suite and verified by footprint assays in vitro. Collectively, this work provides new cues to better understand the detailed regulatory networks of QS systems. ChIP-seq of 10 QS regulators in Pseudomonas aeruginosa

ORGANISM(S): Pseudomonas aeruginosa

SUBMITTER: Xin Deng 

PROVIDER: E-GEOD-65356 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

ChIP-seq reveals the global regulator AlgR mediating cyclic di-GMP synthesis in Pseudomonas aeruginosa.

Kong Weina W   Zhao Jingru J   Kang Huaping H   Zhu Miao M   Zhou Tianhong T   Deng Xin X   Liang Haihua H  

Nucleic acids research 20150722 17


AlgR is a key transcriptional regulator required for the expression of multiple virulence factors, including type IV pili and alginate in Pseudomonas aeruginosa. However, the regulon and molecular regulatory mechanism of AlgR have yet to be fully elucidated. Here, among 157 loci that were identified by a ChIP-seq assay, we characterized a gene, mucR, which encodes an enzyme that synthesizes the intracellular second messenger cyclic diguanylate (c-di-GMP). A ΔalgR strain produced lesser biofilm t  ...[more]

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