Ontology highlight
ABSTRACT:
ORGANISM(S): Homo sapiens
SUBMITTER: Noa Novershtern
PROVIDER: E-GEOD-52824 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Gafni Ohad O Weinberger Leehee L Mansour Abed AlFatah AA Manor Yair S YS Chomsky Elad E Ben-Yosef Dalit D Kalma Yael Y Viukov Sergey S Maza Itay I Zviran Asaf A Rais Yoach Y Shipony Zohar Z Mukamel Zohar Z Krupalnik Vladislav V Zerbib Mirie M Geula Shay S Caspi Inbal I Schneir Dan D Shwartz Tamar T Gilad Shlomit S Amann-Zalcenstein Daniela D Benjamin Sima S Amit Ido I Tanay Amos A Massarwa Rada R Novershtern Noa N Hanna Jacob H JH
Nature 20131030 7479
Mouse embryonic stem (ES) cells are isolated from the inner cell mass of blastocysts, and can be preserved in vitro in a naive inner-cell-mass-like configuration by providing exogenous stimulation with leukaemia inhibitory factor (LIF) and small molecule inhibition of ERK1/ERK2 and GSK3β signalling (termed 2i/LIF conditions). Hallmarks of naive pluripotency include driving Oct4 (also known as Pou5f1) transcription by its distal enhancer, retaining a pre-inactivation X chromosome state, and globa ...[more]