Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Transcription profiling of Saccharomyces cerevisiae treated with enediynes compared to gamma radiation


ABSTRACT: We are investigating the transcriptional response of yeast to treatment with enediynes or gamma radiation, which generate different extents of double or single strand breaks in DNA. We used microarrays to detail the global programme of gene expression underlying the DNA damage response in yeast Experiment Overall Design: Yeast were grown to mid log phase and treated with enediynes or gamma radiation (in biological triplicate) resulting in similar extents of cell killing. The responses were compared to each other and we have deciphered a gene expression profile that is specific for double and single strand breaks in DNA.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Rebecca Fry 

PROVIDER: E-GEOD-5301 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

altmetric image

Publications

The DNA-damage signature in Saccharomyces cerevisiae is associated with single-strand breaks in DNA.

Fry Rebecca C RC   DeMott Michael S MS   Cosgrove Joseph P JP   Begley Thomas J TJ   Samson Leona D LD   Dedon Peter C PC  

BMC genomics 20061212


<h4>Background</h4>Upon exposure to agents that damage DNA, Saccharomyces cerevisiae undergo widespread reprogramming of gene expression. Such a vast response may be due not only to damage to DNA but also damage to proteins, RNA, and lipids. Here the transcriptional response of S. cerevisiae specifically induced by DNA damage was discerned by exposing S. cerevisiae to a panel of three "radiomimetic" enediyne antibiotics (calicheamicin gamma1I, esperamicin A1 and neocarzinostatin) that bind speci  ...[more]

Similar Datasets

2006-10-16 | GSE5301 | GEO
2008-06-16 | E-GEOD-9295 | biostudies-arrayexpress
2014-07-28 | E-GEOD-56622 | biostudies-arrayexpress
2015-12-07 | E-GEOD-59004 | biostudies-arrayexpress
2010-06-24 | E-GEOD-5218 | biostudies-arrayexpress
2018-11-23 | PXD007779 | Pride
2013-03-21 | E-GEOD-36955 | biostudies-arrayexpress
2010-12-30 | E-GEOD-19213 | biostudies-arrayexpress
2010-12-01 | E-MEXP-2679 | biostudies-arrayexpress
2013-07-05 | E-GEOD-39147 | biostudies-arrayexpress