Dataset Information

Dimethyl-Labelling of wild-type yeast treated with hydrogen peroxide LC-MS/MS

ABSTRACT: Changes of protein abundance upon H2O2 treatment of wild-type yeast cells

INSTRUMENT(S): Q Exactive

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Friedel Drepper

LAB HEAD: Bettina Warscheid

PROVIDER: PXD003584 | Pride | 2018-01-24

REPOSITORIES: Pride

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Publications

Quantitative proteomics identifies redox switches for global translation modulation by mitochondrially produced reactive oxygen species.

Topf Ulrike U Suppanz Ida I Samluk Lukasz L Wrobel Lidia L Böser Alexander A Sakowska Paulina P Knapp Bettina B Pietrzyk Martyna K MK Chacinska Agnieszka A Warscheid Bettina B

Nature communications 20180122 1

The generation of reactive oxygen species (ROS) is inevitably linked to life. However, the precise role of ROS in signalling and specific targets is largely unknown. We perform a global proteomic analysis to delineate the yeast redoxome to a depth of more than 4,300 unique cysteine residues in over 2,200 proteins. Mapping of redox-active thiols in proteins exposed to exogenous or endogenous mitochondria-derived oxidative stress reveals ROS-sensitive sites in several components of the translation ...[more]

PMID: 29358734

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