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DNA methylation in postmortem brain samples from autism cases and controls

ABSTRACT: We used the Illumina 450KMethylation BeadChip to measure DNA methylation at 485,512 loci across the genome for 40 postmortem brain samples. The purpose of our study was to identify differentially methylated regions (DMRs) associated with autism. Samples included 16 temporal cortex brain tissue samples (6 cases and 10 controls), 11 prefrontal cortex brain tissue samples (6 cases and 5 controls), and 13 cerebellum brain tissue samples (7 cases and 6 controls). We bisulfute converted DNA from the 40 brain tissue samples, processed, and hybridized them to the Illumina Infinium 450K HumanMethylation450 BeadChip as specificed by the manufacturer.

ORGANISM(S): Homo sapiens

SUBMITTER: Christine Ladd-Acosta

PROVIDER: E-GEOD-53162 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Common DNA methylation alterations in multiple brain regions in autism.

Ladd-Acosta C C Hansen K D KD Briem E E Fallin M D MD Kaufmann W E WE Feinberg A P AP

Molecular psychiatry 20130903 8

Autism spectrum disorders (ASD) are increasingly common neurodevelopmental disorders defined clinically by a triad of features including impairment in social interaction, impairment in communication in social situations and restricted and repetitive patterns of behavior and interests, with considerable phenotypic heterogeneity among individuals. Although heritability estimates for ASD are high, conventional genetic-based efforts to identify genes involved in ASD have yielded only few reproducibl ...[more]

PMID: 23999529

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