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Gene expression changes in a breast cancer stem cell model.

ABSTRACT: Epithelial to mesenchymal transition (EMT) is activated during cancer invasion and metastasis, enriches for cancer stem cells (CSCs), and contributes to therapeutic resistance and disease recurrence. The epithelial cell line MCF7, can be induced to undergo EMT with the induction of PKC by PMA. 5-10% of the resulting cells have a CSC phenotype. This study looks at the transcriptome of these cells and how it differs from cells with a non-CSC phenotype. Epithelial to mesenchymal transition (EMT) is activated during cancer invasion and metastasis, enriches for cancer stem cells (CSCs), and contributes to therapeutic resistance and disease recurrence. The epithelial cell line MCF7, can be induced to undergo EMT with the induction of PKC by PMA. 5-10% of the resulting cells have a CSC phenotype. This study looks at the transcriptome of these cells and how it differs from cells with a non-CSC phenotype. MCF7 cells were stimulated with PMA and FACS sorted. 4 samples, no replicates

ORGANISM(S): Homo sapiens

SUBMITTER: Kristine Hardy 

PROVIDER: E-GEOD-53266 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Chromatinized protein kinase C-θ directly regulates inducible genes in epithelial to mesenchymal transition and breast cancer stem cells.

Zafar Anjum A   Wu Fan F   Hardy Kristine K   Li Jasmine J   Tu Wen Juan WJ   McCuaig Robert R   Harris Janelle J   Khanna Kum Kum KK   Attema Joanne J   Gregory Philip A PA   Goodall Gregory J GJ   Harrington Kirsti K   Dahlstrom Jane E JE   Boulding Tara T   Madden Rebecca R   Tan Abel A   Milburn Peter J PJ   Rao Sudha S  

Molecular and cellular biology 20140602 16

Epithelial to mesenchymal transition (EMT) is activated during cancer invasion and metastasis, enriches for cancer stem cells (CSCs), and contributes to therapeutic resistance and disease recurrence. Signal transduction kinases play a pivotal role as chromatin-anchored proteins in eukaryotes. Here we report for the first time that protein kinase C-theta (PKC-θ) promotes EMT by acting as a critical chromatin-anchored switch for inducible genes via transforming growth factor β (TGF-β) and the key  ...[more]

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