A lack of secretory leukocyte protease inhibitor (SLPI) causes defects in granulocytic differentiation.
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ABSTRACT: Expression data from CD34+ hematopoietic cells transduced with control or anti-SLPI shRNA, serum starved and treated with G-CSF. CD34+ hematopoietic cells were isolated from the bone marrow of healthy individuals, transduced with lenitvirus-based RFP-expressed shRNA constructs targeting SLPI mRNA or scrambled control shRNA. After 3 days of the transductions, RFP+ cells were sorted, serum starved for 24 hours and treated with G-CSF 10 ng/ml for 18 hours. Total RNA of sorted cells was isolated and used for Affymetrix Exome arrays.
ORGANISM(S): Homo sapiens
SUBMITTER: Siarhei Kandabarau
PROVIDER: E-GEOD-53353 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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