Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

A lack of secretory leukocyte protease inhibitor (SLPI) causes defects in granulocytic differentiation.

ABSTRACT: Expression data from CD34+ hematopoietic cells transduced with control or anti-SLPI shRNA, serum starved and treated with G-CSF. CD34+ hematopoietic cells were isolated from the bone marrow of healthy individuals, transduced with lenitvirus-based RFP-expressed shRNA constructs targeting SLPI mRNA or scrambled control shRNA. After 3 days of the transductions, RFP+ cells were sorted, serum starved for 24 hours and treated with G-CSF 10 ng/ml for 18 hours. Total RNA of sorted cells was isolated and used for Affymetrix Exome arrays.

ORGANISM(S): Homo sapiens

SUBMITTER: Siarhei Kandabarau

PROVIDER: E-GEOD-53353 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

A lack of secretory leukocyte protease inhibitor (SLPI) causes defects in granulocytic differentiation.

Klimenkova Olga O Ellerbeck Wienke W Klimiankou Maksim M Ünalan Murat M Kandabarau Siarhei S Gigina Anna A Hussein Kais K Zeidler Cornelia C Welte Karl K Skokowa Julia J

Blood 20131218 8

We identified diminished levels of the natural inhibitor of neutrophil elastase (NE), secretory leukocyte protease inhibitor (SLPI), in myeloid cells and plasma of patients with severe congenital neutropenia (CN). We further found that downregulation of SLPI in CD34(+) bone marrow (BM) hematopoietic progenitors from healthy individuals resulted in markedly reduced in vitro myeloid differentiation accompanied by cell-cycle arrest and elevated apoptosis. Reciprocal regulation of SLPI by NE is well ...[more]

PMID: 24352879

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Dataset Information

A lack of secretory leukocyte protease inhibitor (SLPI) causes defects in granulocytic differentiation.

Publications

A lack of secretory leukocyte protease inhibitor (SLPI) causes defects in granulocytic differentiation.

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