Unknown,Transcriptomics,Genomics,Proteomics

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Gene expression analysis of Wnt+ and Wnt- effector CD8 T cells


ABSTRACT: Wnt signal transduction during an immune response is involved in the establishment of functional CD8 T cell memory P14 ConductinLacZ CD8 T cells (CD45.2) were transferred in CD45.1+.2+ recipient mice. The day after, recipients were infected with 200pfu LCMV WE. At day 8 after infection, cells from spleen and lymph nodes were harvested, magnetically enriched for CD8 T cells, loaded with the beta-galactosidase substrate (FDG) and sorted as 7AAD-negative CD45.- negative, beta-galactosidase positive versus negative cells. P14 CD8 T cells were sorted at day 8 after LCMV infection according to LacZ activity for RNA extraction and hybridization on Affymetrix microarrays.

ORGANISM(S): Mus musculus

SUBMITTER: Werner Held 

PROVIDER: E-GEOD-53358 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Differences in the transduction of canonical Wnt signals demarcate effector and memory CD8 T cells with distinct recall proliferation capacity.

Boudousquié Caroline C   Danilo Maxime M   Pousse Laurène L   Jeevan-Raj Beena B   Angelov Georgi S GS   Chennupati Vijaykumar V   Zehn Dietmar D   Held Werner W  

Journal of immunology (Baltimore, Md. : 1950) 20140815 6


Protection against reinfection is mediated by Ag-specific memory CD8 T cells, which display stem cell-like function. Because canonical Wnt (Wingless/Int1) signals critically regulate renewal versus differentiation of adult stem cells, we evaluated Wnt signal transduction in CD8 T cells during an immune response to acute infection with lymphocytic choriomeningitis virus. Whereas naive CD8 T cells efficiently transduced Wnt signals, at the peak of the primary response to infection only a fraction  ...[more]

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