Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Transcriptional response of Candida albicans to endothelial cells


ABSTRACT: Candida albicans is exposed to a different host environment during different site of infection. Thus, different virulence factors may be active during differenttypes of infection. However,little is known about the C. albicans genes that are required for the initiation and maintenance of candidiasis. To identify potential virulence factors relevant to hematogenously disseminated candidiasis, we determined the transcriptional response of C. albicans to human umbilical vein endothelial cells (HUVECs) in vitro. Keywords: cell interaction Two different Candida albicans strains, CAI4-URA and a clinical isolate 36082, were used to identify the transcriptional response of C. albicans to HUVECs. The strains were incubated with either the HUVECs or bare plastic for 45, 90, and 180 min. C. albicans RNA was extracted and the transcriptional profile of these organisms was analyzed using the C. albicans oligonucleotide microarray. The transcriptional response to HUVECs was compared to that to bare plastic as a control condition. Each time point contains six biological replicates, three of which are from each C. albicans strain.

ORGANISM(S): Candida albicans

SUBMITTER: Hyunsook Park 

PROVIDER: E-GEOD-5344 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

altmetric image

Publications

Transcriptional responses of candida albicans to epithelial and endothelial cells.

Park Hyunsook H   Liu Yaoping Y   Solis Norma N   Spotkov Joshua J   Hamaker Jessica J   Blankenship Jill R JR   Yeaman Michael R MR   Mitchell Aaron P AP   Liu Haoping H   Filler Scott G SG  

Eukaryotic cell 20090821 10


Candida albicans interacts with oral epithelial cells during oropharyngeal candidiasis and with vascular endothelial cells when it disseminates hematogenously. We set out to identify C. albicans genes that govern interactions with these host cells in vitro. The transcriptional response of C. albicans to the FaDu oral epithelial cell line and primary endothelial cells was determined by microarray analysis. Contact with epithelial cells caused a decrease in transcript levels of genes related to pr  ...[more]

Similar Datasets

2010-06-24 | E-GEOD-5340 | biostudies-arrayexpress
2008-12-01 | GSE5344 | GEO
2010-06-06 | E-GEOD-1620 | biostudies-arrayexpress
2010-06-24 | E-GEOD-20131 | biostudies-arrayexpress
2014-03-30 | E-GEOD-56091 | biostudies-arrayexpress
2008-12-01 | GSE5340 | GEO
2009-06-30 | E-GEOD-12030 | biostudies-arrayexpress
2004-03-03 | E-GEOD-1097 | biostudies-arrayexpress
2014-03-30 | E-GEOD-56092 | biostudies-arrayexpress
2003-12-17 | E-GEOD-918 | biostudies-arrayexpress