Unknown,Transcriptomics,Genomics,Proteomics

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ERK signaling regulates opposing functions of JUN family transcription factors in prostate cancer cell migration


ABSTRACT: Knockdowns of c-JUN and JUND had opposite effects on PC3 prostate cell migration. We predicted that c-JUN and JUND control the same set of cell migration genes, but in opposite directions. To test this hypothesis, mRNA with expression changes in c-JUN and JUND knockdown PC3 cell lines were compared to mRNA levels in control (luciferase knockdown) PC3 cells by RNA-seq. mRNA profiles of luciferase knockdown (WT), c-Jun knockdown, and Jun-D knockdown in PC3 cells were generated using deep sequencing, in triplicate, using Illumina HiSeq. Knockdowns were stable shRNA expression from a lentiviral construct selected with puromycin.

ORGANISM(S): Homo sapiens

SUBMITTER: Peter Hollenhorst 

PROVIDER: E-GEOD-53470 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Extracellular signal-regulated kinase signaling regulates the opposing roles of JUN family transcription factors at ETS/AP-1 sites and in cell migration.

Selvaraj Nagarathinam N   Budka Justin A JA   Ferris Mary W MW   Plotnik Joshua P JP   Hollenhorst Peter C PC  

Molecular and cellular biology 20141020 1


JUN transcription factors bind DNA as part of the AP-1 complex, regulate many cellular processes, and play a key role in oncogenesis. The three JUN proteins (c-JUN, JUNB, and JUND) can have both redundant and unique functions depending on the biological phenotype and cell type assayed. Mechanisms that allow this dynamic switching between overlapping and distinct functions are unclear. Here we demonstrate that JUND has a role in prostate cell migration that is the opposite of c-JUN's and JUNB's.  ...[more]

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