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ETS1 is a genome-wide effector of RAS/ERK signaling in epithelial cells (RNA-Seq)

ABSTRACT: ETS1 and RAS/ERK regulate a common gene expression program in establishing enviroment suitable for prostate cancer cell migration. mRNA profiles of luciferase knockdown (WT), ETS1 knockdown, and U0126 treated DU145 cells were generated using deep sequencing, in triplicate, using Illumina HiSeq. Knockdowns were stable shRNA expression from a lentiviral construct selected with puromycin.

ORGANISM(S): Homo sapiens

SUBMITTER: Joshua Plotnik

PROVIDER: E-GEOD-59020 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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ETS1 is a genome-wide effector of RAS/ERK signaling in epithelial cells.

Plotnik Joshua P JP Budka Justin A JA Ferris Mary W MW Hollenhorst Peter C PC

Nucleic acids research 20141007 19

The RAS/ERK pathway is commonly activated in carcinomas and promotes oncogenesis by altering transcriptional programs. However, the array of cis-regulatory elements and trans-acting factors that mediate these transcriptional changes is still unclear. Our genome-wide analysis determined that a sequence consisting of neighboring ETS and AP-1 transcription factor binding sites is enriched near cell migration genes activated by RAS/ERK signaling in epithelial cells. In vivo screening of candidate ET ...[more]

PMID: 25294825

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ETS1 is a genome-wide effector of RAS/ERK signaling in epithelial cells

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