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Multiple waves of transcriptome changes during extended hypoxic induction in human pulmonary microvascular endothelial cells

ABSTRACT: We profile the expression pattern of human pulmonary microvascular endothelial cells (HPMECs) at different time points of hypoxic stress. Through mRNA-seq, we identify functional waves of minor gene up-regulation at 8 and 24h hypoxia exposure followed by a massive wave of transcriptional activation after 48 hours. By weighted gene co-expression network analysis, we identify hub genes that likely play central roles in hypoxia transcription program. Strikingly, these hub genes included a prominent group of lincRNAs, suggesting non-coding RNAs may also have pivotal roles in the hypoxia regulatory circuit. HPMECs share a core hypoxia signature profile, but with some notably differences, indicating a portion of HPMECs hypoxia response is cell-specific. Collectively, our study comprehensive surveys the hypoxia transcriptome, and provides insight into the temporal dynamics of hypoxia transcriptional response. Time-course expression profiling of HPMECs exposed to hypoxia

ORGANISM(S): Homo sapiens

SUBMITTER: Kevin Huang

PROVIDER: E-GEOD-53510 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Multiple waves of transcriptome changes during extended hypoxic induction in human pulmonary microvascular endothelial cells

Project description:We profile the expression pattern of human pulmonary microvascular endothelial cells (HPMECs) at different time points of hypoxic stress. Through mRNA-seq, we identify functional waves of minor gene up-regulation at 8 and 24h hypoxia exposure followed by a massive wave of transcriptional activation after 48 hours. By weighted gene co-expression network analysis, we identify hub genes that likely play central roles in hypoxia transcription program. Strikingly, these hub genes included a prominent group of lincRNAs, suggesting non-coding RNAs may also have pivotal roles in the hypoxia regulatory circuit. HPMECs share a core hypoxia signature profile, but with some notably differences, indicating a portion of HPMECs hypoxia response is cell-specific. Collectively, our study comprehensive surveys the hypoxia transcriptome, and provides insight into the temporal dynamics of hypoxia transcriptional response.

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