Unknown,Transcriptomics,Genomics,Proteomics

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Analysis of global changes in gene expression induced by human polynucleotide phosphorylase (hPNPaseold-35)

ABSTRACT: Human Polynucleotide Phosphorylase (hPNPaseold-35) is an evolutionarily conserved 3’?5’ exoribonuclease implicated in the regulation of numerous physiological processes like maintenance of mitochondrial homeostasis, mtRNA import and aging-associated inflammation. From an RNase perspective, little is known about the RNA or miRNA species it targets for degradation or whose expression it regulates; except for c-myc and miR-221. To further elucidate the functional implications of hPNPaseold-35 in cellular physiology, we knocked-down and overexpressed hPNPaseold-35 in melanoma and HeLa cells, respectively, and performed gene expression analyses to identify differentially expressed transcripts. Biological triplicates were run on microarrays.

ORGANISM(S): Homo sapiens

SUBMITTER: Catherine Dumur

PROVIDER: E-GEOD-53604 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Analysis of global changes in gene expression induced by human polynucleotide phosphorylase (hPNPase(old-35)).

Sokhi Upneet K UK Bacolod Manny D MD Emdad Luni L Das Swadesh K SK Dumur Catherine I CI Miles Michael F MF Sarkar Devanand D Fisher Paul B PB

Journal of cellular physiology 20141201 12

As a strategy to identify gene expression changes affected by human polynucleotide phosphorylase (hPNPase(old-35)), we performed gene expression analysis of HeLa cells in which hPNPase(old-35) was overexpressed. The observed changes were then compared to those of HO-1 melanoma cells in which hPNPase(old-35) was stably knocked down. Through this analysis, 90 transcripts, which positively or negatively correlated with hPNPase(old-35) expression, were identified. The majority of these genes were as ...[more]

PMID: 24729470

Dataset Information

Analysis of global changes in gene expression induced by human polynucleotide phosphorylase (hPNPaseold-35)

Publications

Analysis of global changes in gene expression induced by human polynucleotide phosphorylase (hPNPase(old-35)).

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