Unknown,Transcriptomics,Genomics,Proteomics

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DNA hypomethylation bypasses the interploidy hybridization barrier in Arabidopsis


ABSTRACT: Plants of different ploidy levels are separated by a strong postzygotic hybridization barrier that is established in the endosperm. Deregulated parent-of-origin specific genes are causal for the response to interploidy hybridizations, revealing an epigenetic basis of this phenomenon. In this study we present evidence that paternal hypomethylation can bypass the interploidy hybridization barrier by alleviating the requirement of the epigenetic Polycomb Repressive Complex 2 (PRC2) in the endosperm. Bypass of the barrier is mediated by suppressed expression of imprinted genes. We show that hypomethylated pollen causes redistribution of CHG methylation to PRC2 target genes, revealing that different epigenetic modifications can functionally substitute for each other. Our work presents a method and the underlying mechanism for the generation of viable triploids, providing an impressive example for the potential of epigenome manipulations for plant breeding. Examination of DNA methylation in Arabidopsis endosperm, embryo, and pollen, and gene expression in seeds

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Juan Santos-Gonzalez 

PROVIDER: E-GEOD-53642 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Hypomethylated pollen bypasses the interploidy hybridization barrier in Arabidopsis.

Schatlowski Nicole N   Wolff Philip P   Santos-González Juan J   Schoft Vera V   Siretskiy Alexey A   Scott Rod R   Tamaru Hisashi H   Köhler Claudia C  

The Plant cell 20140912 9


Plants of different ploidy levels are separated by a strong postzygotic hybridization barrier that is established in the endosperm. Deregulated parent-of-origin specific genes cause the response to interploidy hybridizations, revealing an epigenetic basis of this phenomenon. In this study, we present evidence that paternal hypomethylation can bypass the interploidy hybridization barrier by alleviating the requirement for the Polycomb Repressive Complex 2 (PRC2) in the endosperm. PRC2 epigenetica  ...[more]

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