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CATseq a comparative Arabidopsis Transcriptome - RNA-Seq part

ABSTRACT: A comparative assessment between both technologies, RNASeq and microarrays to detect differential expression in Arabidopsis transcriptome. The sequencing approach use High-throughput sequencing on different Solexa technologies (GAII,HiSeq2000 multiplex or not) Wild type samples were analyzed from 2 tissus (flower buds and leaves) which have a very contrasted transcriptomic profile (i.e very high number of genes differentially expressed). The RNA was extracted from 2 tissus Flower Buds and Leaves from Arabidopsis. The associated GEO series with array part is GSE45345

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Véronique BRUNAUD

PROVIDER: E-GEOD-53673 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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CATseq a comparative Arabidopsis Transcriptome - RNA-Seq part

Project description:A comparative assessment between both technologies, RNASeq and microarrays to detect differential expression in Arabidopsis transcriptome. The sequencing approach use High-throughput sequencing on different Solexa technologies (GAII,HiSeq2000 multiplex or not) Wild type samples were analyzed from 2 tissus (flower buds and leaves) which have a very contrasted transcriptomic profile (i.e very high number of genes differentially expressed).

2014-06-01 | GSE53673 | GEO

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2004-09-01 | E-MEXP-94 | biostudies-arrayexpress

bf_vs_f-Comparative Arabidopsis Transcriptome -Sequencing

Project description:cat-seq - bf_vs_f - A comparative assessment of the Arabidopsis sequencing approach High-throughput sequencing cDNA GAII or HiSeq2000 sequenceur (technology Solexa) and data on CATMA array hybridation (version 6). These different characteristics will be studied through the analysis of control samples but also samples from mutants in genes coding for cytosolic or nuclear PPR proteins - The two selected samples are the flower buds and leaves of Arabidopsis. Two organs known as the transcriptome is very different. This experience will serve as reference 2 dye-swap - organ comparison

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bf_vs_f-Comparative Arabidopsis Transcriptome -Sequencing

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Real-time quantitative PCR analysis of cucumber mitogen activated protein kinase cascade genes in various tissues

Project description:We used Bio-Rad laboratories, lnc. PCR assay panel to analyze expression levels of MAPK genes in various tissues or organs. Cucumber plants of the ‘Jinyan No.4’ cultivar were reared in growth chambers at 28 ± 1 °C with a photoperiod of 16 h light/8 h dark and light intensity of 400 μmol m−2 s−1. The roots, leaves, stems, female flower buds (approximately 3 d before anthesis), male flower buds (approximately 1.0 cm in length), and fruits (10 d after pollination) were collected from flowering plants for tissue expression analysis. The experiment was designed to gain insight into the temporal and spatial transcription patterns of 58 MAPK cascade genes in cucumber.

2015-05-01 | E-GEOD-68438 | biostudies-arrayexpress

Real-time quantitative PCR analysis of cucumber mitogen activated protein kinase cascade genes in various tissues

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Comparative transcriptional profiling between the organs of the scion and rootstock of a homograft (Arabidopsis thaliana)

Project description:Analyses of expression differences in flower bud and leaf of scion and rootstock, in homografts of Arabidopsis Gene expression data of unopened developing flower buds and leaves, newly emerged after homografting, from the scion and the rootstock

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2012-09-30 | GSE34188 | GEO

Degradome analysis in Phalaenopsis aphrodite subsp. formosana

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