Unknown,Transcriptomics,Genomics,Proteomics

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Expression data from hepatitis E virus inoculated PLC/PRF/5 cells

ABSTRACT: Hepatitis E virus (HEV) is an important causative pathogen of acute hepatitis. Because of the absence of an in vitro culture system for HEV, research has been greatly impeded. And interaction between HEV and host cells was mainly studied by tansfection/transinfection system, such as Adeno virus transinfection system. We developed an in vitro culture system for HEV in PLC/PRF/5 cells. With this in vitro culture system, we studied the gene expression profile change by HEV infection. Using a microarray assay, we analysed genes of PLC/PRF/5 cells, whose transcription level could be changed by HEV infection. Five flasks of PLC/PRF/5 cells inoculated with HEV were used as test sample, and five flasks inoculated with serum-free DMEM/199 medium were used as control samples. Both test and control flasks were cultured under the same conditions. Sixty days after inoculation, the test and control flasks was resolved with Trizol and analysed with Affymetrix HG-U133 Plus 2 array.

ORGANISM(S): Homo sapiens

SUBMITTER: Feng Zhang 

PROVIDER: E-GEOD-53731 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Hepatitis E genotype 4 virus from feces of monkeys infected experimentally can be cultured in PLC/PRF/5 cells and upregulate host interferon-inducible genes.

Zhang Feng F   Qi Ying Y   Harrison Tim J TJ   Luo Baobin B   Zhou Yan Y   Li Xiuhua X   Song Aijing A   Huang Weijin W   Wang Youchun Y  

Journal of medical virology 20140711 10

The understanding of the interaction between hepatitis E virus (HEV) and its host cells has been impeded greatly by the absence of a cell culture system. In this study, an efficient cultivation method was developed in PLC/PRF/5 cells for HEV genotype 4 from the feces of monkeys infected experimentally. Compared to minimal essential medium (MEM), mixed Dulbecco's Modified Eagle's Medium (DMEM)/M199 improved the infection efficiency of HEV in PLC/PRF/5 cells. The incubation time and temperature we  ...[more]

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