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Unknown,Transcriptomics,Genomics,Proteomics

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ChIP-chip from lymph node-derived primary lymphocytes with anti-E2F2 and anti-SV40TAg

ABSTRACT: E2F2 is essential for the maintenance of T lymphocyte quiescence. To identify the full set of E2F2 target genes, and to gain further understanding of the role of E2F2 in transcriptional regulation, we have performed ChIP-chip analyses across the genome of lymph node-derived T lymphocytes. Here we show that during quiescence, E2F2 binds the promoters of a large number of genes involved in DNA metabolism and cell cycle regulation, concomitant with their transcriptional silencing. We performed 3 ChIP-chip experiments with an antibody against E2F2 and another 3 ChIP-chip experiments with an antibody against SV40TAg (irrelevant antibody).

ORGANISM(S): Mus musculus

SUBMITTER: Usue Laresgoiti

PROVIDER: E-GEOD-53888 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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E2F2 and CREB cooperatively regulate transcriptional activity of cell cycle genes.

Laresgoiti Usua U Apraiz Aintzane A Olea Miguel M Mitxelena Jone J Osinalde Nerea N Rodriguez José A JA Fullaondo Asier A Zubiaga Ana M AM

Nucleic acids research 20130912 22

E2F2 is essential for the maintenance of T lymphocyte quiescence. To identify the full set of E2F2 target genes, and to gain further understanding of the role of E2F2 in transcriptional regulation, we have performed ChIP-chip analyses across the genome of lymph node-derived T lymphocytes. Here we show that during quiescence, E2F2 binds the promoters of a large number of genes involved in DNA metabolism and cell cycle regulation, concomitant with their transcriptional silencing. A comparison of C ...[more]

PMID: 24038359

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