Role of Fnr1 and Fnr3 in transcriptional regulation under oxygen limitation in Herbaspirillum seropedicae
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ABSTRACT: To determine the direct promoter targets of Fnr proteins, we correlated transcriptional changes observed in single fnr1 and fnr3 mutants (E-MTAB-5741) with ChIP-Seq analysis, taking advantage of C-terminally 3xFlag fnr alleles engineered into the H. seropedicae genome. ChIP-seq data were obtained from cultures grown under limited oxygen availability. Using this approach, DNA-binding targets for the H. seropedicae Fnr1 and Fnr3 proteins were unambiguously revealed and correlated with transcript profiles to determine the specific regulons of each protein.
INSTRUMENT(S): Illumina HiSeq 2000
ORGANISM(S): Herbaspirillum seropedicae
SUBMITTER: Marcelo Batista
PROVIDER: E-MTAB-6395 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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