Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Mycobacterium tuberculosis: Control Vs db-cAMP treated cells

ABSTRACT: Transcriptional profiling of Mycobacterium tuberculosis CDC1551 comparing control butyric acid-treated cells with 20mM db-cAMP treated cells after 2hrs of treatment with shaking at 200rpm at 37C. Two-condition experiment, butyric acid (Control) Vs db-cAMP treated cells (Test). Biological replicates: 3 control, 3 test. One replicate per array.

ORGANISM(S): Mycobacterium tuberculosis

SUBMITTER: Nisheeth Agarwal

PROVIDER: E-GEOD-54289 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Mycobacterium tuberculosis: Control Vs db-cAMP treated cells

Project description:Transcriptional profiling of Mycobacterium tuberculosis CDC1551 comparing control butyric acid-treated cells with 20mM db-cAMP treated cells after 2hrs of treatment with shaking at 200rpm at 37C.

2014-01-23 | GSE54289 | GEO

Deep sequencing analysis of the murine transcriptome response to different virulent parenthood B. melitensis infection

Project description:In this study, we employed the Illumina Genome Analyzer platform to perform a Digital Gene Expression (DGE) analysis of the peritoneal macrophages genome-wide transcriptome response to B.melitensis infection. Common changes in gene expression were observed among Brucella app. infected macrophages suggesting similar strategies were employed for their survival and replication, inducing anti-inflammatory and anti-apoptotic. A total of 1019 differentially expressed (DE) transcripts were identified in the macrophages 4 hours after differ virluent B.melitensis infection, especially genes participated lysosome pathway and MAPK pathway. Our findings demonstrate previously unrecognized changes in gene transcription that are associated with B.melitensis infection in the macrophages, and many significant pathways (cascades) identified in the study clearly merit further investigation. Our data provide new clues to understand the molecular attenuation mechanism of M5-90 and a firm foundation for reducing vaccine residual virulence, enhancing vaccine efficacy. Examination oftwo peritoneal macrophages infected brucella and one blank control.

2012-12-01 | E-GEOD-26337 | biostudies-arrayexpress

Expression data from cAMP-treated WT or IFN-gR1-deficient T cells

Project description:cAMP inhibits TCR signaling, T cell proliferation, cytokine production and T cell function. We used microarrays to detail the global programme of gene expression in TCR-activated WT or IFN-gR1-deficient CD4+ T cells by db-cAMP. CD4+ T cells were purified from spleens of WT or IFN-gR1-deficient mice by autoMACS, and activated with anti-CD3 plus anti-CD28 for 15 h. db-cAMP or vehicle control (med) was added into cultures for the last 3 h.

2012-07-23 | E-GEOD-39592 | biostudies-arrayexpress

Expression data from cAMP-treated WT or IFN-gR1-deficient T cells

2012-07-24 | GSE39592 | GEO

Host response to Bifidobacterium bifidum PRL2010 tretment as determined through global transcription profiling

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2013-11-19 | E-GEOD-48533 | biostudies-arrayexpress

Gene expression comparison of P. atrosepticum and it's derivative vasH mutant

Project description:Effect of mutagenesis of a sigma 54-dependent transcriptional regulator encoding gene vasH (ECA3425) to global gene expression in P. atrosepticum SCRI1043 in planta mimicking conditions. Three biological replicates of the wild-type strain SCRI1043 and the mutant strain M-NM-^TvasH were grown in hrp inducing minimal media supplemented with 10% v/v tuber extract untill the culture reached late exponential phase. Differences in the gene expression in SCRI1043 and M-NM-^TvasH were analyzed by microarray.

2013-07-03 | E-GEOD-48471 | biostudies-arrayexpress

Mycobacterium tuberculosis

Project description:Mycobacterium tuberculosis: Control Vs db-cAMP treated cells

| PRJNA236135 | ENA

Functional genomic analyses of Bifidobacterium bifidum PRL2010 highlight how this microorganism interact with the human host

Project description:Bifidobacteria represents one of the dominant group of microorganisms colonizing the intestine of infants. However, the genetic determinants supporting the establishment and the interaction with the human hosts are still largely unknown. Most commensal bacteria interacting with eukaryotic hosts express adhesive molecules on their surfaces that modulate interaction with host cell receptors or with soluble macromolecules. Whole genome transcription profiling of B. bifidum PRL2010, a strain isolated from infant stool, under in vitro as well as in vivo conditions revealed the expression of few common extracellular proteins among which type 1 pili encoding genes. To investigate the molecular mechanisms sustaining the interaction of PRL2010 strain with the human gut, we first explored the global genome transcription profiling of this strain in a in vitro human model such as in the presence of HT29 cell lines. The transcriptome was analyzed using a custom B. bifidum PRL2010 array representing the 90% of this organismM-bM-^@M-^Ys protein coding genes. To better evaluate the conserved responses by B. bifidum, the in vivo transcriptomes were quantified against a diverse set of transcriptome patterns identified for in vitro laboratory cultures of the strain, i.e., B. bifidum responses after growth on an cellM-bM-^@M-^Ys monolayers growth medium (DMEM); B.bifidum responses after growth on synthetic medium (MRS). Briefly, we analized five conditions, two of which are also used as references. Every experiment was performed in duplicate and in vivo condition was performed in quadruplicate.

2013-07-01 | E-GEOD-36442 | biostudies-arrayexpress

EDL933 cultured in bovine small intestine content (BSIC) vs M9 minimal media

Project description:Enterohemorrhagic Escherichia coli (EHEC) are transmitted from cattle to human by means of contaminated food products resulting from fecal contamination. Transcriptome analysis was performed to gain further insight into the metabolic pathways required for persistence and growth of EHEC in the bovine intestine. Understanding the physiology of EHEC in the gut of ruminants is critical to identifying the potential nutritional basis to limiting EHEC shedding. A global transcriptome analysis was performed to gain further insight into the metabolic pathways required for persistence and growth of EHEC in the bovine intestine. DNA microarrays were performed using RNA from EHEC O157:H7 EDL933 incubated in bovine small intestine content (BSIC) compared with cells incubated in M9-minimal media. Four biological replicates collected for bacterial cultures on separate days for each media and labelled following a dye-switch design : For each media two replicates labeled in Cy3 and two replicates in Cy5.

2013-08-01 | E-GEOD-49468 | biostudies-arrayexpress

Differentially regulated genes induced in Mycobacterium tuberculosis by in vitro acid-nitrosative multi-stress

Project description:Transcriptional profile of Mycobacterium tuberculosis in in vitro acid-nitrosative multistress, comparing untreated control cells and bacteria under multi-stress. Two-condition experiment, MTB-K vs. MTB-ACID-NO treated. Biological replicates: 3 controls, 3 Acid-NO treated, independently grown and harvested. In controls and Acid-NO treated cells, good quality RNA samples obtained from each pellet were pooled together and used for microarray experiments to get an average of three separate experiments, five replicates per array.

2013-11-05 | E-GEOD-34623 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data