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Differentially regulated genes induced in Mycobacterium tuberculosis by in vitro acid-nitrosative multi-stress

ABSTRACT: Transcriptional profile of Mycobacterium tuberculosis in in vitro acid-nitrosative multistress, comparing untreated control cells and bacteria under multi-stress. Two-condition experiment, MTB-K vs. MTB-ACID-NO treated. Biological replicates: 3 controls, 3 Acid-NO treated, independently grown and harvested. In controls and Acid-NO treated cells, good quality RNA samples obtained from each pellet were pooled together and used for microarray experiments to get an average of three separate experiments, five replicates per array.

ORGANISM(S): Mycobacterium tuberculosis H37Rv

SUBMITTER: Andrea Cossu

PROVIDER: E-GEOD-34623 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Expression profiling of Mycobacterium tuberculosis H37Rv and Mycobacterium smegmatis in acid-nitrosative multi-stress displays defined regulatory networks.

Cossu Andrea A Sechi Leonardo Antonio LA Bandino Ennio E Zanetti Stefania S Rosu Valentina V

Microbial pathogenesis 20131101

Several studies regarding the transcriptome of Mycobacterium tuberculosis following the exposure to various in vitro simulated phagosomal stressors, have already tried to elucidate the bacterium behavior during the intracellular infection. An in vitro acid-nitrosative multi-stress was carried out for M. tuberculosis H37Rv and Mycobacterium smegmatis MC(2)155 in order to analyze by DNA-microarray the gene expression changes associated respectively to pathogenic and non-pathogenic mycobacterial sp ...[more]

PMID: 24184341

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