Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Serpinb3a contributes to early skin inflammation following allergen exposure

ABSTRACT: Serpinb3a knockout mice show attenuated Transepidermal Water Loss (TEWL) and S100A8 expression (an early inflammatory marker) following treatment with Aspergillus fumigatus extract. The aim of the RNAseq experiments was to provided mechanistic insight for the role of Serpinb3a by identifying early differences in gene expression following allergen exposure. Wild type Balb/c and Serpinb3a null mouse backs were shaved and 24 hours later a patch containing 200ug of Aspergillus fumigatus extract was applied to the skin. After 3 days the patch was removed and the skin was harvested after an additional 24 hours.

ORGANISM(S): Mus musculus

SUBMITTER: Mario Medvedovic

PROVIDER: E-GEOD-54640 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

SERPINB3/B4 contributes to early inflammation and barrier dysfunction in an experimental murine model of atopic dermatitis.

Sivaprasad Umasundari U Kinker Kayla G KG Ericksen Mark B MB Lindsey Mark M Gibson Aaron M AM Bass Stacey A SA Hershey Nicolas S NS Deng Jingyuan J Medvedovic Mario M Khurana Hershey Gurjit K GK

The Journal of investigative dermatology 20140811 1

Serine proteases are critical for epidermal barrier homeostasis, and their aberrant expression and/or activity is associated with chronic skin diseases. Elevated levels of the serine protease inhibitors SERPINB3 and SERPINB4 are seen in patients with atopic dermatitis and psoriasis. However, their mechanistic role in the skin is unknown. To evaluate the contribution of Serpinb3a (mouse homolog of SERPINB3 and SERPINB4) in atopic dermatitis, we examined the effect of topical Aspergillus fumigatus ...[more]

PMID: 25111616

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Project description:Genes involved in the inflammatory response resulting in allergic contact dermatitis (ACD) are only partly known. In this study, we introduce the use of high density oligonucleotide arrays for gene expression profiling in human skin during the elicitation of ACD. Skin biopsies from normal and nickel-exposed skin were obtained from 7 nickel-allergic patients and 5 non-allergic controls at four different time points during elicitation of eczema: 0h, 7h, 48h and 96h. Each gene expression profile was analysed by hybridization to high density oligonucletide arrays. Cluster analysis of 74 genes found to be differentially expressed in the patients over time revealed that the patient samples may be categorised into two groups: An early time point group (0h and 7h) and a late time point group (48h and 96h). Compared to the early time points, late time point skin samples were characterised by the up-regulation of inflammatory molecules including genes involved in the class I antigen presenting pathway and genes involved in lymphocyte adhesion and motility ; Correspondence analysis including both patients and controls revealed three distinct groups: i) the control group, ii) the early time point patient group (0h and 7h) and iii) the late time point patient group (48h and 96h). Experiment Overall Design: For the microarray study, 7 nickel-allergic patients and 5 non-allergic controls were recruited. All subjects were female. The skin covering the upper nates was exposed to nickel delivered during a patch test. Skin biopsies were taken to generate a time-series. Skin was exposed to nickel for 0h, 7h, 48h and 96h. Experiment Overall Design: All nickel allergic patients reacted with visible eczama at the 48h and 96h time-points. No eczema was vissible at the 0h or 7h time-points. Control subjects did not show visible eczema at any time-point. Experiment Overall Design: RNA was extracted from the skin biopsies and 34 biopsies yielded RNA of sufficient quantity and quality for micro-array analysis.

Dataset Information

Serpinb3a contributes to early skin inflammation following allergen exposure

Publications

SERPINB3/B4 contributes to early inflammation and barrier dysfunction in an experimental murine model of atopic dermatitis.

Similar Datasets

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