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Transcription profiling of human peripheral blood derived monocytes, LPS stimulation time-series

ABSTRACT: To evaluate gene expression in human peripheral blood derived monocytes over the course of an LPS stimulation time-series. Experiment Overall Design: Blood samples from three individuals, each sample split into separate culture for each timepoint (0, 2, 4, 8, and 24 hours). RNA prepared from all, and analyzed with Bioanalyzer. Two best RNA samples from each timepoint analyzed by hybridization to a chip.

ORGANISM(S): Homo sapiens

SUBMITTER: Jared Roach

PROVIDER: E-GEOD-5504 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Statistical analysis of MPSS measurements: application to the study of LPS-activated macrophage gene expression.

Stolovitzky G A GA Kundaje A A Held G A GA Duggar K H KH Haudenschild C D CD Zhou D D Vasicek T J TJ Smith K D KD Aderem A A Roach J C JC

Proceedings of the National Academy of Sciences of the United States of America 20050124 5

Massively Parallel Signature Sequencing (MPSS), a recently developed high-throughput transcription profiling technology, has the ability to profile almost every transcript in a sample without requiring prior knowledge of the sequence of the transcribed genes. As is the case with DNA microarrays, effective data analysis depends crucially on understanding how noise affects measurements. We analyze the sources of noise in MPSS and present a quantitative model describing the variability between repl ...[more]

PMID: 15668391

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