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NAC45 and NAC86 direct sieve element differentiation that culminates in enucleation

ABSTRACT: Comparison of the expression profiles of nac45-2 nac86 mutant with col WT, and comparison of the expression profiles of NAC45 extopical induction with non-induction. Three-dye gene expression study with three biological replicates. Five-point time-series experiment and double-mutant vs wild-type comparison.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Panu Somervuo

PROVIDER: E-GEOD-55140 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Plant development. Arabidopsis NAC45/86 direct sieve element morphogenesis culminating in enucleation.

Furuta Kaori Miyashima KM Yadav Shri Ram SR Lehesranta Satu S Belevich Ilya I Miyashima Shunsuke S Heo Jung-ok JO Vatén Anne A Lindgren Ove O De Rybel Bert B Van Isterdael Gert G Somervuo Panu P Lichtenberger Raffael R Rocha Raquel R Thitamadee Siripong S Tähtiharju Sari S Auvinen Petri P Beeckman Tom T Jokitalo Eija E Helariutta Ykä Y

Science (New York, N.Y.) 20140731 6199

Photoassimilates such as sugars are transported through phloem sieve element cells in plants. Adapted for effective transport, sieve elements develop as enucleated living cells. We used electron microscope imaging and three-dimensional reconstruction to follow sieve element morphogenesis in Arabidopsis. We show that sieve element differentiation involves enucleation, in which the nuclear contents are released and degraded in the cytoplasm at the same time as other organelles are rearranged and t ...[more]

PMID: 25081480

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