Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Developmental Profile of DNA Methylation in the Developing Heart

ABSTRACT: Background: To better understand the role DNA methylation plays in regulating gene expression in the developng heart and furthermore the role it plays in heart development we performed genome wide DNA methylation profiling of embryonic hearts at embryonic day (E)11.5 and E14.5 using methyl sensitive tiny fragment enrichment coupled with massive parallel sequencing by using the methyl-sensitive restriction enzyme HpyCH4IV, recognition site 'ACGT'. Results: We found that global methylation remains stable at analyzed 'ACGT' sites (1.64 million site) in developing hearts between E11.5 and E14.5. However, differential methylation was identified at individual loci enriched at genes involved in heart development suggesting a role for DNA methylation in the developing heart. Used Methyl Sensitive Tiny Fragment Enrichment/Massive Parallel Sequencing (MSFE/MPS) to assay methylation at 'ACGT' sites throughout the genome and generate a developmental profile of DNA methylation in the embryonic heart and to identify differences between developing mouse hearts at E11.5 and E14.5.

ORGANISM(S): Mus musculus

SUBMITTER: mingyan lin 

PROVIDER: E-GEOD-55141 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Json Xml
altmetric image

Publications

DNA methylation is developmentally regulated for genes essential for cardiogenesis.

Chamberlain Alyssa A AA   Lin Mingyan M   Lister Rolanda L RL   Maslov Alex A AA   Wang Yidong Y   Suzuki Masako M   Wu Bingruo B   Greally John M JM   Zheng Deyou D   Zhou Bin B  

Journal of the American Heart Association 20140619 3

<h4>Background</h4>DNA methylation is a major epigenetic mechanism altering gene expression in development and disease. However, its role in the regulation of gene expression during heart development is incompletely understood. The aim of this study is to reveal DNA methylation in mouse embryonic hearts and its role in regulating gene expression during heart development.<h4>Methods and results</h4>We performed the genome-wide DNA methylation profiling of mouse embryonic hearts using methyl-sensi  ...[more]

Similar Datasets

Methylation profiling Developmental Profile of DNA Methylation in the Developing Heart
2014-06-27 | GSE55141 | GEO
Genomics Genome wide hydroxymethylation tested using the HELP-GT assay shows redistribution in cancer
2013-07-30 | E-GEOD-42723 | biostudies-arrayexpress
Genomics Peripheral blood mononuclear cells HELP-tagging cytosine methylation data (Albert Einstein College of Medicine)
2014-04-14 | E-GEOD-43705 | biostudies-arrayexpress
Genomics Liver tissue HELP-tagging cytosine methylation data from C57BL/6J mice receiving high-fat diet with or without lingonberries
2015-09-20 | E-GEOD-67277 | biostudies-arrayexpress
Genomics Loss of MEN1 activates DNMT1 implicating DNA hypermethylation as a driver of MEN1 tumorigenesis
2015-12-22 | E-GEOD-64412 | biostudies-arrayexpress
Genomics HEK 293T cells HELP-tagging cytosine methylation data treated with 5-aza-2-deoxycytidine
2015-02-13 | E-GEOD-62592 | biostudies-arrayexpress
Genomics Tet1 is a tumor suppressor of hematopoietic malignancy
2015-04-12 | E-GEOD-65895 | biostudies-arrayexpress
Genomics Human embryonic stem cells HELP-tagging cytosine methylation data (Albert Einstein College of Medicine)
2010-04-12 | E-GEOD-19937 | biostudies-arrayexpress
Genomics Genome-wide methylation and expression analysis of two breast cancer cell lines [Methyl-MAPS]
2013-06-15 | E-GEOD-45336 | biostudies-arrayexpress
Transcriptomics Quantitative Proteomic Profiling of Murine Embryonic Heart Development Reveals a Role for the Mevalonate Pathway in Cardiomyocyte Proliferation [Heart_RNAseq]
2023-04-07 | GSE223237 | GEO