Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Effect of chronic valproic acid treatment on hepatic gene expression profile in Wfs1 knockout mouse.

ABSTRACT: Aim of the present study was to compare the effect of chronic VPA treatment in wild type and Wfs1 knockout mice on hepatic gene expression profile. Wild type, Wfs1 heterozygous and homozygous mice were treated with VPA for three months (300 mg/kg i.p. daily) and gene expression profiles in liver were evaluated using Affymetrix Mouse GeneChip 1.0 st arrays. male wild-type and Wfs1 mutant (+/- and -/-) mice were treated with valproic acid (300 mg/kg/day i.p.) or vehicle (saline, 10ml/kg i.p.) for 12 weeks starting from age 4 to 6 weeks. At age 16 to 18 weeks mice were killed, RNA extracted from the liver and analysed using Affymetrix Mouse Gene 1.0 ST Arrays. Expression of few genes was verified using RT-PCR. There were 8 animals in every group, 48 animals total.

ORGANISM(S): Mus musculus

SUBMITTER: Anton Terasmaa

PROVIDER: E-GEOD-55143 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Effect of chronic valproic acid treatment on hepatic gene expression profile in Wfs1 knockout mouse.

Project description:Aim of the present study was to compare the effect of chronic VPA treatment in wild type and Wfs1 knockout mice on hepatic gene expression profile. Wild type, Wfs1 heterozygous and homozygous mice were treated with VPA for three months (300 mg/kg i.p. daily) and gene expression profiles in liver were evaluated using Affymetrix Mouse GeneChip 1.0 st arrays.

2014-02-20 | GSE55143 | GEO

VPA alleviates neurological deficits and restores gene expression in a mouse model of Rett syndrome

Project description:Rett syndrome (RTT) is a devastating neurodevelopmental disorder that occurs once in every 10,000-15,000 live female births. Despite intensive research, no effective cure is yet available. Valproic acid (VPA) has been used widely to treat mood disorder, epilepsy, and a growing number of other disorders. In limited clinical studies, VPA has also been used to control seizure in RTT patients with promising albeit somewhat unclear efficacy. In this study we tested the effect of VPA on the neurological symptoms of RTT and discovered that short-term VPA treatment during the symptomatic period could reduce neurological symptoms in RTT mice. We found that VPA restores the expression of a subset of genes in RTT mouse brains, and these genes clustered in neurological disease and developmental disorder networks. Our data suggest that VPA could be used as a drug to alleviate RTT symptoms. Wild type or MeCP2KO mice received daily injections of VPA (300 mg/kg) for 2 weeks. Each experimental condition: WT control, KO treated with VPA (KO+VPA), and KO treated with saline (KO+saline). Half brain samples were retrieved.

2014-10-04 | E-GEOD-56780 | biostudies-arrayexpress

Transcription profiling of liver from Wistar rats treated with either pentadecafluoro-octanoic acid (PFOA) or 1H,1H,2H,2H-heptadecafluorodecan-1-ol (8:2 FTOH)

Project description:Wistar rats were treated with either PFOA or 8:2 FTOH for 10 days with the following doses, 3, 10 or 25 mg/kg Bwt, followed by one recovery day. The treatment was given daily by i.p. injections. The rats were humanly sacrificed by decapitation, and the liver was immediatly dissected and divided, one piece of the liver was sumerged in RNALater (for use in microarray and realtime experiments) and the other piece of liver was submerged in cold buffer(for ezymatic assays). Pentadecafluorooctanoic (Perfluorooctanoic acid, PFOA) acid is an industrial surfactant. 8:2 FTOH is a fluorotelomer alcohol.

2007-04-10 | E-MEXP-1045 | biostudies-arrayexpress

microRNAs profiling in hippocampal cells of mouse with Pilocarpine-induced Status Epilepticus (SE)

Project description:SE was induced in mice (NMRI) chemically by a single dose of Pilocarpine (300mg/kg;i.p.). RNA was extracted from the hippocampi of treated and wild-type mice at 24 h and 28 d after induction. microRNA expressions were measured via microarray technology using Exiqon's miCURY™ LNA Arrays. 4 conditions, Pilocarpine treated mice at 24 h and 28 d, and the control wild-type at the same types, each of 8 replicates.

2014-01-01 | E-GEOD-51841 | biostudies-arrayexpress

Protein microarray analysis of expression of murine cytokines in spleen interfered with IFN-α and (or) LPS

Project description:12 wild-type C57BL/6 (B6) mice were divided into 4 groups: control group, IFN-α group, LPS group, and IFN-α+ LPS group, every group contained 3 mice (n=3). IFN-α was administrated i.p. to IFN-α group and IFN-α+ LPS group once daily (QD) for 7 days at a medium dose of 105units/kg weight, PBS was administrated i.p. to control group and LPS group QD for 7 days at the same volume. LPS group and IFN-α+ LPS group were injected i.v. with 10 μg LPS for one mouse on the 8th day. Control group and IFN-α group were injected i.v. with PBS at the same volume. 6 h later, mice were sacrificed to harvest spleens for protein microarray experiment. Mouse Cytokine Antibody Array 3(62) was purchased from Ray Biotech, Norcross GA, US. Protein microarray of murine cytokines expression. Spleens from 12 mice (4 group) were treated as indicated in the summary. Equal amount total protein from each spleen was pooled prior to gene expression analysis.

2012-11-10 | E-GEOD-42183 | biostudies-arrayexpress

Transcription profiling of mouse livers of adult and newborn animal response to Aflatoxin B1

Project description:Susceptibility to the hepatocarcinogen Aflatoxin B1 (AFB1) varies among species and with age. Mice are refractory to carcinogenic and toxic effects of AFB1; however, B6C3F1 mice show transient sensitivity if dosed shortly after birth. We compared age-related differences in gene expression and transcriptional responses to AFB1 in livers of newborn (4-day-old) and adult mice. Experiment Overall Design: Experiments were carried out under the protocol approved by MIT Animal Care Committee. Pregnant C57BL/6J female mice, mated to C3H/HeJ males, and adult (at least 60 day-old) B6C3F1/J hybrid F1 male mice (C57BL/6J female x C3H/HeJ male) were obtained from Jackson Labs. They were housed in plastic cages (one per cage) under controlled environmental conditions and 12 h light/dark cycle. Food and water were supplied ad libitum. After female mice gave birth, the male B6C3F1 pups were dosed at 4 days by a single i.p. injection of AFB1 (6 mg/kg body weight) in DMSO (10 ml/kg body weight) or the same volume of just DMSO. The treatments were always performed at the same time of the day and the pups were returned to their mothers until they were sacrificed 4 or 24 h after treatment. Adult B6C3F1 males were treated with the same doses and volumes of AFB1 or DMSO after an acclimation period of at least two weeks and sacrificed 4 or 24 hours after treatment. We noticed that injection of a volume of 10 ml/kg (whether AFB1 or vehicle alone) is tolerated poorly by adult animals and thus another group of adult animals was added which was treated with the same (6 mg/kg) dose of AFB in lower (5 ml/kg) volume. Corresponding vehicle control animals (5ml/kg DMSO) were also used. In addition, untreated controls were used and sacrificed together with the treated animals. Three independant bilogical replicates were used for each experimental point. Total RNA was isolated from livers and processed according to standard Affymetrix protocol.

2009-01-14 | E-GEOD-4740 | biostudies-arrayexpress

TCDD induced gene expression changes in dendritic cells

Project description:Effect of the over activation of the aryl hydrocarbon receptor on gene expression of spleen derived dendritic cells. 8-12 week old, female C57BL6 mice were injected 10 Âµg/kg TCDD i.p. or solvent control. After 24 h mice were sacrificed and splenic dendritic cells purified by MACS sorting.

2008-11-25 | E-GEOD-13719 | biostudies-arrayexpress

Role of AhR in thymocyte emigration in vivo

Project description:Over-activation of the aryl hydrocarbon receptor by TCDD in mice leads among other phenotypes to a severe thymic atrophy accompanied by immunosuppression. TCDD causes a block in thymocyte maturation and a preferential emigration of immature CD4-CD8- DN thymocytes (recent thymic emigrants) into the periphery. As part of this study gene expression profiles from DN thymocytes and thymic emigrants were generated from TCDD and solvent control mice Keywords: Affymetrix, TCDD, CD4-CD8- thymocytes, Thymus involution, thymic emigration, Ahr Female, 6-8 week old C57BL6 mice were i.p. injected with 10 M-BM-5g/kg TCDD. After 5 days mice were anesthezised and FITC injected into their thymi. after 24h mice were sacrificed and CD4-CD8-FITC+ cells isolated from thymus (thymocytes) and spleen (recent thymus emigrants).

2008-11-25 | E-GEOD-10166 | biostudies-arrayexpress

Global gene expression profiles of MT knockout and wild-type mice in the condition of doxorubicin-induced cardiomyopathy

Project description:The present study was designed using MT knockout mice in concert with genomic approaches to explore the possible molecular and cellular mechanisms involved in the protective effects of MT against DOX cardiotoxicity. MT-M-bM-^EM- /M-bM-^EM-! null (MT-/-) mice and corresponding wild-type mice (MT+/+) were administrated with a single dose of DOX (15 mg/kg, i.p.) or an equal volume of saline. Animals were sacrificed on the 4th day after DOX administration and samples were collected for further analyses. Global gene expression profiles of cardiac mRNA from two genotype mice revealed that 381 characteristically MT-responsive genes were identified between MT+/+ mice and MT-/- mice in response to DOX, including fos, ucp3, car3, atf3, map3k6, etc.. Functional analysis implied MAPK signaling pathway, p53 signaling pathway, Jak-STAT signaling pathway, PPAR signaling pathway, Wnt signaling pathway, etc. might be involved to mediate the protection of DOX cardiomyopathy by MT. Results from the present study not only validated the previously reported possible mechanisms of MT protection against DOX toxicity, but also provided new clues into the molecular mechanisms involved in this process. Male MT+/+ & MT-/- mice (6-8 weeks old) were randomly assigned to treatment or control groups and administrated with a single dose of DOX (15 mg/kg, i.p.) or an equal volume of normal saline solution (NS) respectively. Animals were sacrificed on the 4th day after DOX injection, and samples were collected for further microarray analyses.

2013-08-10 | E-GEOD-23598 | biostudies-arrayexpress

Transcription profiling of mouse poloxamer P-407 chronic hyperlipidemia mouse model

Project description:We performed a systemic study of the poloxamer P-407-induced chronic hyperlipidemia in C57BL/6J mice. Mice were treated 96 days every third day.The poloxamer mostly elevates plasma triglycerides and cholesterol while change of the liver cholesterol is not significant. Experiment Overall Design: A group of 12 mice were administered 0.5 g/kg poloxamer P-407 by intraperitoneal (i.p.) injection (poloxamere group), a control group of 4 mice were injected every third day with normal saline. Total RNA from P-407 treated 12 mice has been pooled into 3 biological replicas (Smpl1, Smpl2 and Smpl3). Reference total RNA has been obtained by pooling 4 mice from control group. Each RNA sample has been splitted and hybridised in parallel on cDNA (G4104A) and oligonucleotide (G4120A) mouse microarrays (Agilent Technologies, CA, USA.

2008-06-14 | E-GEOD-6305 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data