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Genomewide analysis of QKI-dependent alternative splicing

ABSTRACT: To identify QKI targets, we performed QKI knockdown in BEAS2B cells and analyzed alternative splicing patterns by high-throughput RNA sequencing. The mRNA profiles of control- and QKI-knockdown BEAS2B cells were generated by deep sequencing using Illumina GAIIx sequencer.

ORGANISM(S): Homo sapiens

SUBMITTER: Jingyi Hui

PROVIDER: E-GEOD-55215 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The RNA-binding protein QKI suppresses cancer-associated aberrant splicing.

Zong Feng-Yang FY Fu Xing X Wei Wen-Juan WJ Luo Ya-Ge YG Heiner Monika M Cao Li-Juan LJ Fang Zhaoyuan Z Fang Rong R Lu Daru D Ji Hongbin H Hui Jingyi J

PLoS genetics 20140410 4

Lung cancer is the leading cause of cancer-related death worldwide. Aberrant splicing has been implicated in lung tumorigenesis. However, the functional links between splicing regulation and lung cancer are not well understood. Here we identify the RNA-binding protein QKI as a key regulator of alternative splicing in lung cancer. We show that QKI is frequently down-regulated in lung cancer, and its down-regulation is significantly associated with a poorer prognosis. QKI-5 inhibits the proliferat ...[more]

PMID: 24722255

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