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Genomic Microarray of Rat Alveolar Type 2 cells Following Chronic Ethanol Ingestion

ABSTRACT: Chronic alcohol ingestion changes the alveolar landscape. We used microarrays to characterize the change in mRNA expression following chronic alcohol ingestion in male Sprague Dawley rates (EtOH 36% of calories) We used microarrays to characterize the change in mRNA expression following chronic alcohol ingestion in male Sprague Dawley rats (EtOH 36% of calories for 6 weeks). Control animals were given an isocaloric substitution for EtOH for 6 weeks. Alveolar type 2 cells were isolated using an immuno-absorption /technique and RNA was extracted by Trizol. Four control animals and three EtOH treated animals were used for this study.

ORGANISM(S): Rattus norvegicus

SUBMITTER: Gregory Doho

PROVIDER: E-GEOD-55243 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Chronic ethanol exposure alters the lung proteome and leads to mitochondrial dysfunction in alveolar type 2 cells.

Alli Abdel A AA Brewer Elizabeth M EM Montgomery Darrice S DS Ghant Marcus S MS Eaton Douglas C DC Brown Lou Ann LA Helms My N MN

American journal of physiology. Lung cellular and molecular physiology 20140328 11

The lungs can undergo irreversible damage from chronic alcohol consumption. Herein, we developed an animal model predisposed for edematous lung injury following chronic ingestion of alcohol to better understand the etiology of alcohol-related disorders. Using animal modeling, alongside high-throughput proteomic and microarray assays, we identified changes in lung protein and transcript in mice and rats, respectively, following chronic alcohol ingestion or a caloric control diet. Liquid chromatog ...[more]

PMID: 24682449

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