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Genomic Microarray of Rat Alveolar Type 2 cells Following Chronic Ethanol Ingestion

ABSTRACT: Chronic alcohol ingestion changes the alveolar landscape. We used microarrays to characterize the change in mRNA expression following chronic alcohol ingestion in male Sprague Dawley rates (EtOH 36% of calories)

ORGANISM(S): Rattus norvegicus

PROVIDER: GSE55243 | GEO | 2014/07/02

SECONDARY ACCESSION(S): PRJNA238986

REPOSITORIES: GEO

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Genomic Microarray of Rat Alveolar Type 2 cells Following Chronic Ethanol Ingestion

Project description:Chronic alcohol ingestion changes the alveolar landscape. We used microarrays to characterize the change in mRNA expression following chronic alcohol ingestion in male Sprague Dawley rates (EtOH 36% of calories) We used microarrays to characterize the change in mRNA expression following chronic alcohol ingestion in male Sprague Dawley rats (EtOH 36% of calories for 6 weeks). Control animals were given an isocaloric substitution for EtOH for 6 weeks. Alveolar type 2 cells were isolated using an immuno-absorption /technique and RNA was extracted by Trizol. Four control animals and three EtOH treated animals were used for this study.

2014-07-02 | E-GEOD-55243 | biostudies-arrayexpress

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Project description:In this study, we analyzed the role of miR-21 in liver regeneration after partial hepatectomy (PHx) in chronic ethanol-treated rats. Male Sprague-Dawley rats were fed a liquid diet containing 36% of total calories derived from ethanol for 5 weeks; corresponding pair-fed calorie-matched controls were fed diets in which ethanol calories were replaced by carbohydrate. After 5 weeks, locked nucleic acid (LNA)-modified oligo antisense to miR-21 (AM21, Exiqon, Vedbaek, Denmark) was used to inhibit miRNA in vivo, and rats were subjected to 70% PHx. Liver samples were collected at 24h after the surgery. The excised liver samples at t=0 served as within-animal controls. Rat Gene 2.0 ST (Affymetrix, Santa Clara, CA) arrays were used to obtain global gene expression data from pooled liver samples (pools of 3 or 4 biological replicates/array, total 8 arrays). Gene expression was profiled in AM21 treated or untreated chronic ethanol-fed (EtOH) and carbohydrate control pair-fed (CHO) rat liver prior to (control, t=0) and 24 h after 70% partial hepatectomy (PHx).

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