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Small RNA profile in Moso Bamboo root and leaf


ABSTRACT: The small RNA libraries from Moso bamboo (Phyllostachy heterocycla) roots and leaves were constructed by using high definition adapters . The small RNA profiles were analyzed. A collection of micro RNAs with similarity to the micro RNA entries in mirbase were discovered. The putative genomic loci of the micro RNAs were identified. Analysis of small RNA profiles from the root and leaf tissues of young Moso Bamboo seedlings

ORGANISM(S): Phyllostachys edulis

SUBMITTER: Irina Mohorianu 

PROVIDER: E-GEOD-55305 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Small RNA profile in moso bamboo root and leaf obtained by high definition adapters.

Xu Ping P   Mohorianu Irina I   Yang Li L   Zhao Hansheng H   Gao Zhimin Z   Dalmay Tamas T  

PloS one 20140731 7


Moso bamboo (Phyllostachy heterocycla cv. pubescens L.) is an economically important fast-growing tree. In order to gain better understanding of gene expression regulation in this important species we used next generation sequencing to profile small RNAs in leaf and roots of young seedlings. Since standard kits to produce cDNA of small RNAs are biased for certain small RNAs, we used High Definition adapters that reduce ligation bias. We identified and experimentally validated five new microRNAs  ...[more]

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