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In vivo proximity labeling and sequencing (IPL-seq) for SNRPN70 in 293T-Rex

ABSTRACT: This proof-of-principle experiment was designed to demonstrate the feasibility of proximity labeling for RNAM-bM-^@M-^Sprotein interactions IPL-seq on 293T-Rex expressing MSA-SNRPN70 (sample) or NFH-SNRPN70 (control)

ORGANISM(S): Homo sapiens

SUBMITTER: Roberto Bonasio

PROVIDER: E-GEOD-55370 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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In vivo proximity labeling for the detection of protein-protein and protein-RNA interactions.

Beck David B DB Narendra Varun V Drury William J WJ Casey Ryan R Jansen Pascal W T C PW Yuan Zuo-Fei ZF Garcia Benjamin A BA Vermeulen Michiel M Bonasio Roberto R

Journal of proteome research 20141029 12

Accurate and sensitive detection of protein-protein and protein-RNA interactions is key to understanding their biological functions. Traditional methods to identify these interactions require cell lysis and biochemical manipulations that exclude cellular compartments that cannot be solubilized under mild conditions. Here, we introduce an in vivo proximity labeling (IPL) technology that employs an affinity tag combined with a photoactivatable probe to label polypeptides and RNAs in the vicinity o ...[more]

PMID: 25311790

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