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Identification of tRNA sequences in Lactococcus lactis

ABSTRACT: we report the identification and sequences of the tRNAome of industrially relevant microorganism Lactococcus lactis Three Next Generation sequencing runs annotated as S1, S2 and S3 were performed. Cells were harvested at exponential phase and tRNA was isolated. S1 and S2 were spiked with Phe-tRNAGAA from yeast and Lys-tRNAUUU from E. coli prior to cell lysis. S3 was spiked with Phe-tRNAGAA from yeast and Lys-tRNAUUU from E. coli before the library preparation to estimate the possible loss of tRNA in the extraction process.

ORGANISM(S): Lactococcus lactis subsp. cremoris MG1363

SUBMITTER: Gong Zhang

PROVIDER: E-GEOD-55392 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Systematic identification of tRNAome and its dynamics in Lactococcus lactis.

Puri Pranav P Wetzel Collin C Saffert Paul P Gaston Kirk W KW Russell Susan P SP Cordero Varela Juan A JA van der Vlies Pieter P Zhang Gong G Limbach Patrick A PA Ignatova Zoya Z Poolman Bert B

Molecular microbiology 20140806 5

Transfer RNAs (tRNAs) through their abundance and modification pattern significantly influence protein translation. Here, we present a systematic analysis of the tRNAome of Lactococcus lactis. Using the next-generation sequencing approach, we identified 40 tRNAs which carry 16 different post-transcriptional modifications as revealed by mass spectrometry analysis. While small modifications are located in the tRNA body, hypermodified nucleotides are mainly present in the anticodon loop, which thro ...[more]

PMID: 25040919

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