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Jmjd3-mediated H3K27me3 dynamics orchestrate brown fat cell fate transition and regulate white fat plasticity

ABSTRACT: We performed ChIP-seq to chart genome-wide maps of H3K27me3 in brown preadipocytes and mature brown adipocytes. We observed a subset of brown fat-specific genes, but not common fat genes or white fat-specific genes, possess the H3K27me3 mark in preadipocytes, and this mark is erased in mature adipocytes. H3K27me3 ChIP-seq in brown preadipocytes and mature adipocytes.

ORGANISM(S): Mus musculus

SUBMITTER: Yongxu Wang

PROVIDER: E-GEOD-55469 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Jmjd3-Mediated H3K27me3 Dynamics Orchestrate Brown Fat Development and Regulate White Fat Plasticity.

Pan Dongning D Huang Lei L Zhu Lihua J LJ Zou Tie T Ou Jianhong J Zhou William W Wang Yong-Xu YX

Developmental cell 20151125 5

Progression from brown preadipocytes to adipocytes engages two transcriptional programs: the expression of adipogenic genes common to both brown fat (BAT) and white fat (WAT), and the expression of BAT-selective genes. However, the dynamics of chromatin states and epigenetic enzymes involved remain poorly understood. Here we show that BAT development is selectively marked and guided by repressive H3K27me3 and is executed by its demethylase Jmjd3. We find that a significant subset of BAT-selectiv ...[more]

PMID: 26625958

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