Unknown,Transcriptomics,Genomics,Proteomics

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High-throughput SuperSAGE for transcript profiling in berry tissues of Vitis vinifera L. under different light regimes

ABSTRACT: SuperSAGE is a method of digital gene expression profiling that allows isolation of 26bp tag fragments from expressed transcripts. Because its tag size is larger than that of conventional SAGE, SuperSAGE allowed a secure tag-to-gene annotation using BLAST search against grape genome databases.Transcript profiles in nine samples of grape berry tissues under different light conditions were obtained by SuperSAGE analysis and used for screening the genes which have co-ordinated transcript profiles with the change in the flavonoid composition in the samples analyzed. Candidate genes related to flavonoid biosynthesis and regulation were identified. Nine different grape samples, i.e., flowers, grape berries of Cabernet Sauvignon at 2, 7, 9 weeks after flowering (WAF), berry skins at 17 days after flowering (DAF) shaded after flowering, and berry skins at 17DAF shaded from flowering to 14DAF and then light exposed, were analyzed.

ORGANISM(S): Vitis vinifera

SUBMITTER: Kazuya Koyama 

PROVIDER: E-GEOD-55473 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Functional characterization of a new grapevine MYB transcription factor and regulation of proanthocyanidin biosynthesis in grapes.

Koyama Kazuya K   Numata Mineyo M   Nakajima Ikuko I   Goto-Yamamoto Nami N   Matsumura Hideo H   Tanaka Nobukazu N  

Journal of experimental botany 20140523 15

A new regulator of proanthocyanidin (PA) biosynthesis in grapes was found by screening genes coordinately expressed with PA accumulation under different light conditions using a substantially improved method of serial analysis of gene expression (SuperSAGE). This R2R3-MYB transcription factor, VvMYBPAR, shows high protein sequence similarity with PA biosynthesis-regulating plant MYBs, such as VvMYBPA2 and TRANSPARENT TESTA2. Its transcript levels were relatively high in the skins of young berrie  ...[more]

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