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Transcription factor Runx1 cooperates with MyoD and c-Jun to regulate the balance of myoblast proliferation/differentiation [Expression Study]

ABSTRACT: Runx1 is expressed in regenrating muscle, specifically in the muscle adult stem cells- the satellite cells. Its exact role and target genes were yet to be identified This experiment aimed at determining the Runx1 regulated genes in muscle regeneration, specifically in Satellite cells To elucidate the Runx1-mediated transcriptional program involved in early stages of muscle regeneration, we compared gene expression profiles in WT and Runx1f/f primary myobalsts (PM). PM were purified from both genotypes , Total RNA was isolated, reverse-transcribed, fragmented, labeled and hybridized to Affymetrix MoGene 1.0 ST DNA array.

ORGANISM(S): Mus musculus

SUBMITTER: kfir Umansky

PROVIDER: E-GEOD-55556 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcription factor Runx1 cooperates with MyoD and c-Jun to regulate the balance of myoblast proliferation/differentiation [ATAC-Seq]

Project description:Runx1 is expressed in regenrating muscle, specifically in the muscle adult stem cells- the satellite cells. Its exact role and target genes were yet to be identified. We report here the genome wide open chromatin paatern, as established by ATAC-seq Examination ofgenome wide pattern naked and therefore trhansposase accecible genomic DNA in primary myoblasts (PM)

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Transcription factor Runx1 cooperates with MyoD and c-Jun to regulate the balance of myoblast proliferation/differentiation [ChIP-Seq Histone]

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Transcription factor Runx1 cooperates with MyoD and c-Jun to regulate the balance of myoblast proliferation/differentiation [ChIP-seq]

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Transcription factor Runx1 cooperates with MyoD and c-Jun to regulate the balance of myoblast proliferation/differentiation [ATAC-Seq]

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Transcription factor Runx1 cooperates with MyoD and c-Jun to regulate the balance of myoblast proliferation/differentiation [ChIP-Seq Histone]

Project description:Runx1 is expressed in regenrating muscle, specifically in the muscle adult stem cells- the satellite cells. Its exact role and target genes were yet to be identified. We report here the genome wide histone modification using an active enhancer pattern of H3K4 monomethylation (H3K4me1) and H3K27 acetylation (H3K27ac).

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