Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Identification of stress response and recovery mechanisms by multi-scale analyses of drought effect in the maize leaf growth zone.

ABSTRACT: We use the gowth zone of the maize leaf as a model system to study the growth reduction in response to drought stress. The spatial gradient and the relatively large size of the maize leaf allowed us to sample at a subzonal rezolution and to examine different developmental stages at the same time. We compared the response to different levels of drought stress (mild and severe) of proliferating (meristem), expanding (elongation zone) and differentiated (mature zone) tissue. Three separate loop designs were used for the three zones of the maize leaf (meristem, elongation zone, and mature zone). In each loop three treatments were contrasted (control, mild stress, and severe stress). Four biological replicates were used for each zone/condition (4 replicates x 3 zones x 3 conditions = 36 samples).

ORGANISM(S): Zea mays

SUBMITTER: Gerrit Beemster

PROVIDER: E-GEOD-55592 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Drought Induces Distinct Growth Response, Protection, and Recovery Mechanisms in the Maize Leaf Growth Zone.

Avramova Viktoriya V AbdElgawad Hamada H Zhang Zhengfeng Z Fotschki Bartosz B Casadevall Romina R Vergauwen Lucia L Knapen Dries D Taleisnik Edith E Guisez Yves Y Asard Han H Beemster Gerrit T S GT

Plant physiology 20150821 2

Drought is the most important crop yield-limiting factor, and detailed knowledge of its impact on plant growth regulation is crucial. The maize (Zea mays) leaf growth zone offers unique possibilities for studying the spatiotemporal regulation of developmental processes by transcriptional analyses and methods that require more material, such as metabolite and enzyme activity measurements. By means of a kinematic analysis, we show that drought inhibits maize leaf growth by inhibiting cell division ...[more]

PMID: 26297138

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Project description:Switchgrass plants were grown in a Sandwich tube system to induce gradual drought stress by withholding watering. After 29 days, leaf photosynthetic rate decreased significantly, compared to the control plants which were watered regularly. The drought-treated plants recovered to the same leaf water content after three days of re-watering. Root tip (1cm basal fragment, designated as RT1 hereafter) and the elongation/maturation zone (the next upper 1 cm tissue, designated as RT2 hereafter) tissues were collected at the 29th day of drought stress treatment, (named SDT for severe drought treated), one (D1W) and three days (D3W) of re-watering. The tandem mass tags mass spectrometry-based quantitative proteomics analysis was performed to identify the proteomes, and drought-induced differentially expressed proteins (DEPs). From RT1 tissues, 6,156, 7,687 and 7,699 proteins were quantified, and 296, 535 and 384 DEPs were identified in the SDT, D1W and D3W samples, respectively. From RT2 tissues, 7,382, 7,255 and 6,883 proteins were quantified, and 393, 587 and 321 proteins DEPs were identified in the SDT, D1W and D3W samples. Between RT1 and RT2 tissues, very few DEPs overlapped at SDT, but the number of such proteins increased during the recovery phase. A large number of hydrophilic proteins and stress-responsive proteins were induced during SDT and remained at a higher level during the recovery stages. A large number of DEPs in RT1 tissues maintained the same expression pattern throughout drought treatment and the recovery phases. The DEPs in RT1 tissues were classified in cell proliferation, mitotic cell division, and chromatin modification, and those in RT2 were placed in cell wall remodeling and cell expansion processes. This study provided information pertaining to root zone-specific proteome changes during drought and recover phases, which will allow us to choose the proteins (genes) as better defined targets for developing drought tolerant plants.

Dataset Information

Identification of stress response and recovery mechanisms by multi-scale analyses of drought effect in the maize leaf growth zone.

Publications

Drought Induces Distinct Growth Response, Protection, and Recovery Mechanisms in the Maize Leaf Growth Zone.

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