Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Identification of Angustifolia 3 (ZmAN3) target genes through GSyellow-based ChIP-seq in Zea mays leaves

ABSTRACT: Genome-wide target genes of ZmAN3 were identified through ChIP-seq on the growth zone of the maize leaf, encompassing the division, transition and expansion zone. For ChIP-seq, ZmAN3 was fused to the GSyellow TAP tag and expressed from the ubiquitin promoter (pUBIL). The pUBIL:ZmAN3-GSyellow construct was transformed into the maize inbred line B104. ChIP was performed using anti-GFP antibody (abcam290).

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Zea mays

SUBMITTER: Geert De Jaeger

PROVIDER: E-MTAB-6527 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

GS<sup>yellow</sup>, a Multifaceted Tag for Functional Protein Analysis in Monocot and Dicot Plants.

Besbrugge Nienke N Van Leene Jelle J Eeckhout Dominique D Cannoot Bernard B Kulkarni Shubhada R SR De Winne Nancy N Persiau Geert G Van De Slijke Eveline E Bontinck Michiel M Aesaert Stijn S Impens Francis F Gevaert Kris K Van Damme Daniel D Van Lijsebettens Mieke M Inzé Dirk D Vandepoele Klaas K Nelissen Hilde H De Jaeger Geert G

Plant physiology 20180420 2

The ability to tag proteins has boosted the emergence of generic molecular methods for protein functional analysis. Fluorescent protein tags are used to visualize protein localization, and affinity tags enable the mapping of molecular interactions by, for example, tandem affinity purification or chromatin immunoprecipitation. To apply these widely used molecular techniques on a single transgenic plant line, we developed a multifunctional tandem affinity purification tag, named GS<sup>yellow</sup ...[more]

PMID: 29678859

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Dataset Information

Identification of Angustifolia 3 (ZmAN3) target genes through GSyellow-based ChIP-seq in Zea mays leaves

Publications

GS<sup>yellow</sup>, a Multifaceted Tag for Functional Protein Analysis in Monocot and Dicot Plants.

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