Ontology highlight
ABSTRACT:
ORGANISM(S): Mus musculus
SUBMITTER: Francesco Neri
PROVIDER: E-GEOD-55660 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Neri Francesco F Incarnato Danny D Krepelova Anna A Rapelli Stefania S Anselmi Francesca F Parlato Caterina C Medana Claudio C Dal Bello Federica F Oliviero Salvatore S
Cell reports 20150205 5
Ten eleven translocation (Tet) proteins oxidize 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), and 5-carboxylcytosine (5caC). 5fC and 5caC can be further excised by thymine-DNA glycosylase (Tdg). Here, we present a genome-wide approach, named methylation-assisted bisulfite sequencing (MAB-seq), that enables single-base resolution mapping of 5fC and 5caC and measures their abundance. Application of this method to mouse embryonic stem cells (ESCs) shows the occur ...[more]