Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Single base resolution analysis of 5-formyl and 5-carboxyl cytosine reveals the actual promoter DNA methylation dynamics in embryonic stem cells


ABSTRACT: This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

ORGANISM(S): Mus musculus

SUBMITTER: Francesco Neri 

PROVIDER: E-GEOD-55660 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

altmetric image

Publications

Single-Base Resolution Analysis of 5-Formyl and 5-Carboxyl Cytosine Reveals Promoter DNA Methylation Dynamics.

Neri Francesco F   Incarnato Danny D   Krepelova Anna A   Rapelli Stefania S   Anselmi Francesca F   Parlato Caterina C   Medana Claudio C   Dal Bello Federica F   Oliviero Salvatore S  

Cell reports 20150205 5


Ten eleven translocation (Tet) proteins oxidize 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), and 5-carboxylcytosine (5caC). 5fC and 5caC can be further excised by thymine-DNA glycosylase (Tdg). Here, we present a genome-wide approach, named methylation-assisted bisulfite sequencing (MAB-seq), that enables single-base resolution mapping of 5fC and 5caC and measures their abundance. Application of this method to mouse embryonic stem cells (ESCs) shows the occur  ...[more]

Similar Datasets

2015-02-10 | E-GEOD-55658 | biostudies-arrayexpress
2014-11-02 | E-GEOD-62631 | biostudies-arrayexpress
2013-05-06 | E-GEOD-44350 | biostudies-arrayexpress
2014-08-21 | E-GEOD-55566 | biostudies-arrayexpress
2013-05-06 | E-GEOD-43298 | biostudies-arrayexpress
2008-07-06 | E-GEOD-11034 | biostudies-arrayexpress
2014-08-21 | E-GEOD-55568 | biostudies-arrayexpress
2015-09-10 | E-GEOD-72606 | biostudies-arrayexpress
2015-06-22 | E-GEOD-65784 | biostudies-arrayexpress
2014-11-14 | E-GEOD-60334 | biostudies-arrayexpress