Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

41 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Expression data from RECA1-overexpressing plants

ABSTRACT: Arabidopsis nuclear RecA homologue RA51D were reported to be involved in plant defense responses. Plant organelles such as mitochondria and chloroplast also have their own RecA homologues. We focused on chloroplast RecA homologue RECA1 because it has been well known that the precursors of phytohormones and secondary metabolites related to plant defense responses are synthesized in chloroplast and recent studies have identified several chloroplastic proteins invoved with plant defense responses. We used microarrays to investigate the global gene expression changes by RECA1 and identified the up-regualted genes involved with plant defense response. 2-week-old Col-0 and RECA1(at1g79050)-overexpressing plants without any treatments were used for RNA extraction and hybridization on Affymetrix microarrays. Col-0 plants were used as control and T3 RECA1-overexpressing transgenic plants were used in this study.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Hyesung Jeon

PROVIDER: E-GEOD-55913 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Expression data from RECA1-overexpressing plants

Project description:Arabidopsis nuclear RecA homologue RA51D were reported to be involved in plant defense responses. Plant organelles such as mitochondria and chloroplast also have their own RecA homologues. We focused on chloroplast RecA homologue RECA1 because it has been well known that the precursors of phytohormones and secondary metabolites related to plant defense responses are synthesized in chloroplast and recent studies have identified several chloroplastic proteins invoved with plant defense responses. We used microarrays to investigate the global gene expression changes by RECA1.

2014-10-15 | GSE55913 | GEO

Microarray analysis of rice ZH11 and OPR3 overexpression plants

Project description:The plant hormone jasmonic acid (JA) plays a central role in plant defense responses.12-oxo-phytodienoic acid (OPDA) reductase 3 (OPR3) is a key enzyme in the JA synthesis pathway.To discover what facilitates defense functions in the JA pathway, we conducted a microarray analysis of OPR3 overexpressing rice plants (OPR3ox) and the wild-type Zhonghua 11.

2015-06-20 | E-MTAB-3425 | biostudies-arrayexpress

Effect of Serendipita indica-infection on the gene expression in wild-type and cbl7 knockout mutants Arabidopsis thaliana plants

Project description:The root-colonizing fungal endophyte Serendipita indica, formerly known as Piriformospora indica, is well known to promote plant biomass production and stress tolerance of its host plants. Previous studies highlighted an important role of calcium Ca2+ signaling in the establishment of the plant–fungus interaction. We here report the comparative analysis of the effect of a mock- and S. indica-infection on both wild-type Arabidopsis plants (Col-3) and cbl7 knockout mutants. Our data provide evidence for the involement of the Ca2+ sensor CBL7 in the control of potassium distribution in the plant and in adjusting plant defense responses to allow the establishment of the plant–fungus symbiosis. The impairment of CBL7 was shown to translate into increased induction of plant defense-related genes.

2023-09-05 | GSE242292 | GEO

Jasmonate-responsive genes in rice

Project description:The plant hormone jasmonate (JA) plays important roles in the regulation of defense responses in many plants. To clarify the response to JA in rice at gene expression level, we performed a microarray analysis using the Agilent Rice Oligo Microarray (44k, custom-made; Agilent Technologies, Redwood City, CA, USA). As a result, treatment of JA caused high upregulation of many defense-related genes including pathogenesis-related (PR) genes in rice. However, many of these defense-related genes were not upregulated in JA-insensitive transgenic rice plant overexpressing JAZ8deltaC.

2012-05-30 | GSE35429 | GEO

Plant Natriuretic Peptides induce a specific set of proteins diagnostic for an adaptive response to abiotic stress

Project description:In plants, structural and physiological evidence has suggested the presence of biologically active natriuretic peptides (PNPs). PNPs are secreted into the apoplast, are systemically mobile and elicit a range of responses signaling via cGMP. The PNP-dependent responses include tissue specific modifications of cation transport and changes in stomatal conductance and the photosynthetic rate. PNP also has a critical role in host defense responses. Surprisingly, PNP-homologues are also produced by several plant pathogens during host colonization suppressing host defense responses. Here we show that a synthetic peptide representing the biologically active fragment of the Arabidopsis thaliana PNP (AtPNP-A) induces the production of reactive oxygen species in suspension-cultured A. thaliana (Col-0) cells.

2014-11-20 | PXD001386 | Pride

Gene expression profiling shows that a jaz decuple mutation (jazD) promotes expression of defense-related genes while inhibiting expression of growth-related genes

Project description:The decuple jaz mutation (jazD) affects plant growth and defense. We used RNA-sequencing to query the transcriptomes of a jaz quintuple mutant (jazQ) and jazD, and examined how these mutations alter the expression of growth- and defense-associated genes in comparison to wild-type Col-0 plants. The data highlight how jasmonate pathways contribute to growth and defense.

2018-11-20 | GSE116681 | GEO

Gene expression profiling shows that CYCLIN-DEPENDENT KINASE 8 (CDK8) promotes the expression of defense-related genes in a higher-order jaz (jazD) mutant

Project description:The decuple jaz mutation (jazD) promotes defense responses while inhibiting growth. We used RNA-sequencing to query the the transcriptomes of wild-type Col-0, cdk8-1, jazD and jazD cdk8-1 plants. The data highlight how CDK8 contributes to the expression of growth- and defense-related genes in jazD.

2023-03-19 | GSE147226 | GEO

The corepressor of Topless-related 1 (TPR1): Transcript analysis of TPR1-GFP lines

Project description:Transcriptional corepressors of the Topless family are important regulators of plant hormone and immunity signaling. We present here the transcriptome of Arabidopsis thaliana Col-0 plants expressing GFP-tagged TPR1 (Topless-related 1). Expression of TPR1 driven by its own promoter (pTPR1:TPR1-GFP) in Arabidopsis results in EDS1-dependent defense activation and dwarfed growth affected by the plant immune regulator EDS1 but not by accumulation of salicylic acid. We used total RNA sequencing to characterize the transcriptional status of TPR1-GFP expressing lines in Col, eds1-2 and sid2-1 mutant backgrounds.

2021-06-29 | GSE154652 | GEO

Long-term growth under elevated CO2 differentially suppresses biotic stress genes in non-acclimated versus cold-acclimated winter wheat

Project description:This study compared the photosynthetic performance and the global gene expression of the winter hardy wheat Triticum aestivum cv Norstar grown under non-acclimated (NA) or cold-acclimated (CA) condition at either ambient CO2 or elevated CO2 (EC). CA Norstar maintained comparable light saturated and CO2 saturated rates of photosynthesis but lower quantum requirements for photosystem II and non photochemical quenching relative to NA plants even at EC. Neither NA nor CA plants were sensitive to feedback inhibition of photosynthesis at EC. Global gene expression using microarray combined with bioinformatics analysis revealed that genes affected by EC were 3 times higher in NA (1022 genes) compared to CA (372 genes) Norstar. The most striking effect was the down-regulation of genes involved in the plant defense responses in NA Norstar. In contrast, cold acclimation reversed this down regulation due to the cold induction of genes involved in plant pathogenesis resistance, and cellular and chloroplast protection. These results suggest that EC have less impact on plant performance and productivity in cold adapted winter hardy plants in the northern climates compared to warmer environments. Selection for cereal cultivars with constitutively higher expression of biotic stress defense genes may be necessary under EC during the warm growth period and in warmer climates.

2013-08-23 | GSE48620 | GEO

Expression of a dominant‐negative AtNEET‐H89C protein disrupts iron–sulfur metabolism and iron homeostasis in Arabidopsis

Project description:Iron–sulfur (Fe–S) clusters play an essential role in plants as protein cofactors mediating diverse electron transfer reactions. Because they can react with oxygen to form reactive oxygen species (ROS) and inflict cellular damage, the biogenesis of Fe–S clusters is highly regulated. A recently discovered group of 2Fe–2S proteins, termed NEET proteins, was proposed to coordinate Fe–S, Fe and ROS homeostasis in mammalian cells. Here we report that disrupting the function of AtNEET, the sole member of the NEET protein family in Arabidopsis thaliana, triggers leaf‐associated Fe–S‐ and Fe‐deficiency responses, elevated Fe content in chloroplasts (1.2–1.5‐fold), chlorosis, structural damage to chloroplasts and a high seedling mortality rate. Our findings suggest that disrupting AtNEET function disrupts the transfer of 2Fe–2S clusters from the chloroplastic 2Fe–2S biogenesis pathway to different cytosolic and chloroplastic Fe–S proteins, as well as to the cytosolic Fe–S biogenesis system, and that uncoupling this process triggers leaf‐associated Fe–S‐ and Fe‐deficiency responses that result in Fe over‐accumulation in chloroplasts and enhanced ROS accumulation. We further show that AtNEET transfers its 2Fe–2S clusters to DRE2, a key protein of the cytosolic Fe–S biogenesis system, and propose that the availability of 2Fe–2S clusters in the chloroplast and cytosol is linked to Fe homeostasis in plants.

2019-12-22 | GSE127300 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data