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Differential gene expression in erythroid progenitor cells from ?-thalassaemia patients and healthy controls [BRB-ArrayTools analysis]

ABSTRACT: This study uses microarray technology to examine the erythroid progenitor mRNA of patients with transfusion dependent ?-thalassaemia and compare it to erythroid progenitor mRNA from healthy controls. We observed no statistical difference in gene expression between the groups following 7 days in culture. However, following 14 days in culture we observed differential expression of 277 genes. Haematopoietic cells from the peripheral blood of 6 ?-thalassaemia patients and 6 healthy controls was grown in semi-solid media. After 7 and 14 days in culture cells of erythroid origin were isolated. Total RNA was isolated from these for microarray gene expression analysis

ORGANISM(S): Homo sapiens

SUBMITTER: Luke Forster

PROVIDER: E-GEOD-56088 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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