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Effect of TTF-1/NKX2-1 expression on TGF-beta induced gene expression in A549 lung cancer cell line.

ABSTRACT: TTF-1/NKX2-1 was expressed by adenoviral vector and changes in gene expression were determined by RNA-sequencing. A549 cells were infected with Ad-TTF-1 or Ad-LacZ vectors and stimulated with TGF-beta for 24 hours or left untreated. Expression of polyA RNA was determined.

ORGANISM(S): Homo sapiens

SUBMITTER: Daizo Koinuma

PROVIDER: E-GEOD-56356 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A Smad3 and TTF-1/NKX2-1 complex regulates Smad4-independent gene expression.

Isogaya Kazunobu K Koinuma Daizo D Tsutsumi Shuichi S Saito Roy-Akira RA Miyazawa Keiji K Aburatani Hiroyuki H Miyazono Kohei K

Cell research 20140725 8

Thyroid transcription factor-1 (TTF-1, also known as NKX2-1) is a tissue-specific transcription factor in lung epithelial cells. Although TTF-1 inhibits the epithelial-to-mesenchymal transition induced by transforming growth factor-β (TGF-β) in lung adenocarcinoma cells, the mechanism through which TTF-1 inhibits the functions of TGF-β is unknown. Here we show that TTF-1 disrupts the nuclear Smad3-Smad4 complex without affecting the nuclear localization of phospho-Smad3. Genome-wide analysis by ...[more]

PMID: 25060702

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