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Identification of targets regulated by MLL1 in response to HSP90 inhibitors

ABSTRACT: MLL1 WT or KO MEF with and without HSP90 inhibitor treatment MEF cells were seeded in 6-well plates for 3d with 100ng/ml doxycycline prior to treatment with 0.1% DMSO or 100nM AUY922 for 3h

ORGANISM(S): Mus musculus

SUBMITTER: yaoyu chen

PROVIDER: E-GEOD-56492 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Identification of mixed lineage leukemia 1(MLL1) protein as a coactivator of heat shock factor 1(HSF1) protein in response to heat shock protein 90 (HSP90) inhibition.

Chen Yaoyu Y Chen Jinyun J Yu Jianjun J Yang Guizhi G Temple Emilia E Harbinski Fred F Gao Hui H Wilson Christopher C Pagliarini Raymond R Zhou Wenlai W

The Journal of biological chemistry 20140515 27

Heat shock protein 90 (HSP90) inhibition inhibits cancer cell proliferation through depleting client oncoproteins and shutting down multiple oncogenic pathways. Therefore, it is an attractive strategy for targeting human cancers. Several HSP90 inhibitors, including AUY922 and STA9090, show promising effects in clinical trials. However, the efficacy of HSP90 inhibitors may be limited by heat shock factor 1 (HSF1)-mediated feedback mechanisms. Here, we identify, through an siRNA screen, that the h ...[more]

PMID: 24831003

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